• Western blot analysis of extracts of various cell lines, using DDAH2 Rabbit monoclonal antibody (STJ11102007) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DDAH2 antibody (1-100) [S7MR] (STJ11102007)

SKU:
STJ11102007

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-DDAH2 (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDAH2
Gene ID: 23564
Uniprot ID: DDAH2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DDAH2 (O95865).
Immunogen Sequence: MGTPGEGLGRCSHALIRGVP ESLASGEGAGAGLPALDLAK AQREHGVLGGKLRQRLGLQL LELPPEESLPLGPLLGDTAV IQGDTALITRPWSPARRPEV
Tissue Specificity Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.
Function Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Protein Name N(G -N(G-Dimethylarginine Dimethylaminohydrolase 2
Ddah-2
Dimethylarginine Dimethylaminohydrolase 2
Ddahii
Dimethylargininase-2
Protein G6a
S-Phase Protein
Database Links Reactome: R-HSA-203615
Cellular Localisation Cytoplasm
Mitochondrion
Translocates From Cytosol To Mitochondrion Upon Il-1beta Stimulation In Chondrocytes
Alternative Antibody Names Anti-N(G -N(G-Dimethylarginine Dimethylaminohydrolase 2 antibody
Anti-Ddah-2 antibody
Anti-Dimethylarginine Dimethylaminohydrolase 2 antibody
Anti-Ddahii antibody
Anti-Dimethylargininase-2 antibody
Anti-Protein G6a antibody
Anti-S-Phase Protein antibody
Anti-DDAH2 antibody
Anti-DDAH antibody
Anti-G6A antibody
Anti-NG30 antibody

Information sourced from Uniprot.org

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