| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-DCP1A (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000. WB: Approx 75kDa band observed in lysates of cell line A431 and HeLa (calculated MW of 63.3kDa according to NP_060873.3). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | DCP1A |
| Gene ID: | 55802 |
| Uniprot ID: | DCP1A_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_060873.3 |
| Immunogen Sequence: | QVLTKNKDNHN |
| Function | Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1. Essential for embryonic development. |
| Protein Name | Mrna-Decapping Enzyme 1a Smad4-Interacting Transcriptional Co-Activator Transcription Factor Smif |
| Database Links | Reactome: R-HSA-430039 Reactome: R-HSA-450385 Reactome: R-HSA-450513 Reactome: R-HSA-975957 |
| Cellular Localisation | Cytoplasm P-Body Nucleus Co-Localizes With Nanos3 In The Processing Bodies Predominantly Cytoplasmic In Processing Bodies (Pb) Nuclear After Tgfb1 Treatment Translocation To The Nucleus Depends On Interaction With Smad4 |
| Alternative Antibody Names | Anti-Mrna-Decapping Enzyme 1a antibody Anti-Smad4-Interacting Transcriptional Co-Activator antibody Anti-Transcription Factor Smif antibody Anti-DCP1A antibody Anti-SMIF antibody |
Information sourced from Uniprot.org
