• Western blot analysis of extracts of various cell lines, using DCN antibody (STJ117242) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of extracts of HeLa cells, using DCN antibody (STJ117242) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using DCN rabbit polyclonal antibody (STJ117242) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using DCN rabbit polyclonal antibody (STJ117242) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human testis using DCN rabbit polyclonal antibody (STJ117242) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using DCN rabbit polyclonal antibody (STJ117242) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DCN antibody (31-359) (STJ117242)

SKU:
STJ117242

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DCN (31-359) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DCN
Gene ID: 1634
Uniprot ID: PGS2_HUMAN
Immunogen Region: 31-359
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 31-359 of human DCN (NP_001911.1).
Immunogen Sequence: DEASGIGPEVPDDRDFEPSL GPVCPFRCQCHLRVVQCSDL GLDKVPKDLPPDTTLLDLQN NKITEIKDGDFKNLKNLHAL ILVNNKISKVSPGAFTPLVK LERLYLSKNQLKELPEKMPK TLQELRAHENEITKVRKVTF NGLNQMIVIELGTNPLKSSG IENGAFQGMKKLSYIRIADT NITSIPQGLPPSLTELHLDG NKISRVDAASLKGLNNLAKL GLSFNSISAVDNGSLANTP
Tissue Specificity Detected in placenta (at protein level). Detected in cerebrospinal fluid, fibroblasts and urine (at protein level).
Post Translational Modifications The attached glycosaminoglycan chain can be either chondroitin sulfate or dermatan sulfate depending upon the tissue of origin.
Function May affect the rate of fibrils formation.
Protein Name Decorin
Bone Proteoglycan Ii
Pg-S2
Pg40
Database Links Reactome: R-HSA-1474228
Reactome: R-HSA-1971475
Reactome: R-HSA-2022870
Reactome: R-HSA-2022923
Reactome: R-HSA-2024101
Reactome: R-HSA-3000178
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3595172
Reactome: R-HSA-3595174
Reactome: R-HSA-3595177
Reactome: R-HSA-4420332
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Decorin antibody
Anti-Bone Proteoglycan Ii antibody
Anti-Pg-S2 antibody
Anti-Pg40 antibody
Anti-DCN antibody
Anti-SLRR1B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance