Anti-DCLRE1B antibody (C-Term) (STJ96429)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the C-terminal region of human SNM1B.
STJ96429
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General Information

Short DescriptionRabbit polyclonal anti-5 exonuclease Apollo (C-Term) for use in WB, ELISA and IHC in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA/IHC
HostRabbit
ReactivityHuman/Rat/Mouse
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
IHC-P 1:50-300
ELISA 2000-20000
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolDCLRE1B
Gene ID64858
Uniprot IDDCR1B_HUMAN
ImmunogenSynthesized peptide derived from the C-terminal region of human SNM1B.
Immunogen RegionC-Term
SpecificitySNM1B Polyclonal Antibody detects endogenous levels of SNM1B protein.

Additional Info

Function 5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint. Possesses beta-lactamase activity, catalyzing the hydrolysis of penicillin G and nitrocefin. Exhibits no activity towards other beta-lactam antibiotic classes including cephalosporins (cefotaxime) and carbapenems (imipenem).
Protein Name 5' Exonuclease Apollo
Beta-Lactamase Dclre1b
Dna Cross-Link Repair 1b Protein
Snm1 Homolog B
Snmib
Hsnm1b
Database Links Reactome: R-HSA-6783310
Cellular Localisation Chromosome
Telomere
Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Mainly Localizes To Telomeres
Recruited Via Its Interaction With Terf2
During Mitosis
Localizes To The Centrosome
Alternative Antibody Names Anti-5' Exonuclease Apollo antibody
Anti-Beta-Lactamase Dclre1b antibody
Anti-Dna Cross-Link Repair 1b Protein antibody
Anti-Snm1 Homolog B antibody
Anti-Snmib antibody
Anti-Hsnm1b antibody
Anti-DCLRE1B antibody
Anti-SNM1B antibody

Information sourced from Uniprot.org

Citations

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