| Short Description : | Rabbit polyclonal DBP (1-325) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | DBP |
| Gene ID: | 1628 |
| Uniprot ID: | DBP_HUMAN |
| Immunogen Region: | 1-325 |
| Immunogen Sequence: | MARPVSDRTPAPLLLGGPAG TPPGGGALLGLRSLLQGTSK PKEPASCLLKEKERKAALPA ATTPGPGLETAGPADAPAGA VVGGGSPRGRPGPVPAPGLL APLLWERTLPFGDVEYVDLD AFLLEHGLPPSPPPPGGPSP EPSPARTPAPSPGPGSCGSA SPRSSPGHAPARAALGTASG HRAGLTSRDTPSPVDPDTVE VLMTFEPDPADLALSSIPGH ETFDPRRHRFSEEELKPQP |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human DBP (NP_001343.2). |
| Tissue Specificity | Ubiquitously expressed. Expressed in the suprachiasmatic nuclei (SCN) and in most peripheral tissues, with a strong circadian rhythmicity. |
| Function | This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation. |
| Protein Name | D Site-Binding ProteinAlbumin D Box-Binding ProteinAlbumin D-Element-Binding ProteinTax-Responsive Enhancer Element-Binding Protein 302Taxreb302 |
| Database Links | Reactome: R-HSA-1368108 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-D Site-Binding Protein antibodyAnti-Albumin D Box-Binding Protein antibodyAnti-Albumin D-Element-Binding Protein antibodyAnti-Tax-Responsive Enhancer Element-Binding Protein 302 antibodyAnti-Taxreb302 antibodyAnti-DBP antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and AKR1A1 knockout (KO) 293T cells, using [KO Validated] AKR1A1 Rabbit polyclonal antibody (STJ119987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98772/382820/STJ119987_1__54161.1713151317.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AKR1A1 knockout (KO) 293T cells, using [KO Validated] AKR1A1 Rabbit polyclonal antibody (STJ119987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
