• Western blot analysis of lysates from SH-SY5Y cells, using Dopamine beta Hydroxylase Rabbit monoclonal antibody (STJ11105163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-DBH antibody (100-250) [S5163RM] (STJ11105163)

SKU:
STJ11105163

$197.78 - $863.33
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Dopamine Beta-Hydroxylase (100-250) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5163RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DBH
Gene ID: 1621
Uniprot ID: DOPO_HUMAN
Immunogen Region: 100-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-250 of human Dopamine beta Hydroxylase (NP_000778.3).
Immunogen Sequence: LVVLWTDGDTAYFADAWSDQ KGQIHLDPQQDYQLLQVQRT PEGLTLLFKRPFGTCDPKDY LIEDGTVHLVYGILEEPFRS LEAINGSGLQMGLQRVQLLK PNIPEPELPSDACTMEVQAP NIQIPSQETTYWCYIKELPK GFSRHHIIKYE
Post Translational Modifications N-glycosylated. Proteolytic cleavage after the membrane-anchor leads to the release of the soluble form.
Function Catalyzes the hydroxylation of dopamine to noradrenaline (also known as norepinephrine), and is thus vital for regulation of these neurotransmitters.
Protein Name Dopamine Beta-Hydroxylase
Dopamine Beta-Monooxygenase Cleaved Into - Soluble Dopamine Beta-Hydroxylase
Database Links Reactome: R-HSA-209905
Cellular Localisation Soluble Dopamine Beta-Hydroxylase: Cytoplasmic Vesicle
Secretory Vesicle Lumen
Cytoplasmic Vesicle
Secretory Vesicle
Chromaffin Granule Lumen
Secreted
Secretory Vesicle Membrane
Single-Pass Type Ii Membrane Protein
Chromaffin Granule Membrane
Alternative Antibody Names Anti-Dopamine Beta-Hydroxylase antibody
Anti-Dopamine Beta-Monooxygenase Cleaved Into - Soluble Dopamine Beta-Hydroxylase antibody
Anti-DBH antibody

Information sourced from Uniprot.org

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