• Western blot analysis of extracts of various cell lines, using DARS2 antibody (STJ110123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded human liver damage using DARS2 antibody (STJ110123) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using DARS2 antibody (STJ110123) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using DARS2 antibody (STJ110123) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Anti-DARS2 antibody (406-645) (STJ110123)

Anti-DARS2 antibody (406-645) (STJ110123)

SKU:
STJ110123

£112.50 - £524.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DARS2
Gene ID: 55157
Uniprot ID: SYDM_HUMAN
Immunogen Region: 406-645
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 406-645 of human DARS2 (NP_060592.2).
Immunogen Sequence: ANRNWNSPVANFIMESQRLE LIRLMETQEEDVVLLTAGEH NKACSLLGKLRLECADLLET RGVVLRDPTLFSFLWVVDFP LFLPKEENPRELESAHHPFT APHPSDIHLLYTEPKKARSQ HYDLVLNGNEIGGGSIRIHN AELQRYILATLLKEDVKMLS HLLQALDYGAPPHGGIALGL DRLICLVTGSPSIRDVIAFP KSFRGHDLMSNTPDSVPPEE LKPYHIRVSKPTDSKAERA
Function Catalyzes the attachment of aspartate to tRNA(Asp) in a two-step reaction: aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp).
Protein Name Aspartate--Trna Ligase - Mitochondrial
Aspartyl-Trna Synthetase
Asprs
Database Links Reactome: R-HSA-379726
Cellular Localisation Mitochondrion Matrix
Mitochondrion Membrane
Alternative Antibody Names Anti-Aspartate--Trna Ligase - Mitochondrial antibody
Anti-Aspartyl-Trna Synthetase antibody
Anti-Asprs antibody
Anti-DARS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance