• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g DARS antibody (STJ28657). Western blot was performed from the immunoprecipitate using DARS antibody (STJ28657) at a dilution of 1:1000.
  • Immunohistochemistry analysis of DARS in paraffin-embedded mouse brain using DARS Rabbit polyclonal antibody (STJ28657) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DARS in paraffin-embedded mouse spinal cord using DARS Rabbit polyclonal antibody (STJ28657) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of DARS in paraffin-embedded rat brain using DARS Rabbit polyclonal antibody (STJ28657) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using DARS Rabbit polyclonal antibody (STJ28657) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DARS antibody (222-501) (STJ28657)

SKU:
STJ28657

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DARS (222-501) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DARS1
Gene ID: 1615
Uniprot ID: SYDC_HUMAN
Immunogen Region: 222-501
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human DARS (NP_001340.2).
Immunogen Sequence: KIISAASEGGANVFTVSYFK NNAYLAQSPQLYKQMCICAD FEKVFSIGPVFRAEDSNTHR HLTEFVGLDIEMAFNYHYHE VMEEIADTMVQIFKGLQERF QTEIQTVNKQFPCEPFKFLE PTLRLEYCEALAMLREAGVE MGDEDDLSTPNEKLLGHLVK EKYDTDFYILDKYPLAVRPF YTMPDPRNPKQSNSYDMFMR GEEILSGAQRIHDPQLLTER ALHHGIDLEKIKAYIDSFR
Tissue Specificity Expression in the developing and adult brain shows similar patterns. Highly expressed in the ventricular and subventricular zones, including hippocampal subfields, the midlateral temporal cortex and the frontal polar cortex. The cerebellum, cerebral cortex, hippocampus, and lateral ventricle show preferential neuronal expression. Expression in the peripheral neurons is evident in the colon.
Function Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
Protein Name Aspartate--Trna Ligase - Cytoplasmic
Aspartyl-Trna Synthetase
Asprs
Cell Proliferation-Inducing Gene 40 Protein
Database Links Reactome: R-HSA-2408522
Reactome: R-HSA-379716
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Aspartate--Trna Ligase - Cytoplasmic antibody
Anti-Aspartyl-Trna Synthetase antibody
Anti-Asprs antibody
Anti-Cell Proliferation-Inducing Gene 40 Protein antibody
Anti-DARS1 antibody
Anti-DARS antibody
Anti-PIG40 antibody

Information sourced from Uniprot.org

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