| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-Dachshund homolog 1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 75 and 52kDa bands observed in Human Kidney lysates (calculated MW of 73.0kDa according to NP_542937.1 and of 51.9kDa according to NP_004383.2). Recommended concentration: 0.1-0 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | DACH1 |
| Gene ID: | 1602 |
| Uniprot ID: | DACH1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_542937.1; NP_542938.1; NP_004383.2 |
| Specificity: | This antibody is expected to recognise all three reported isoforms (NP_542937.1; NP_542938.1; NP_004383.2). |
| Immunogen Sequence: | SPVENTPQNNECK |
| Function | Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1. Binds to chromatin DNA via its DACHbox-N domain. |
| Protein Name | Dachshund Homolog 1Dach1 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dachshund Homolog 1 antibodyAnti-Dach1 antibodyAnti-DACH1 antibodyAnti-DACH antibody |
Information sourced from Uniprot.org