Host: | Mouse |
Applications: | IHC-P |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Mouse monoclonal antibody anti-Cytokeratin 5 (318-491aa) is suitable for use in Immunohistochemistry research applications. |
Clonality: | Monoclonal |
Clone ID: | ZM186 |
Conjugation: | Unconjugated |
Isotype: | IgG1/Kappa |
Formulation: | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
Purification: | Affinity purified |
Dilution Range: | 1:100‐200 |
Storage Instruction: | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
Gene Symbol: | KRT5 |
Gene ID: | 3852 |
Uniprot ID: | K2C5_HUMAN |
Immunogen Region: | 318-491aa |
Specificity: | Positive control: Prostate |
Immunogen: | Recombinant human Cytokeratin 5 (KRT5) protein fragment (around aa 318-491) |
Tissue Specificity | Expressed in corneal epithelium (at protein level). Expressed in keratinocytes (at protein level). |
Post Translational Modifications | Phosphorylated by CDK1, AURKB and Rho-kinase, phosphorylation is regulated by the cell cycle. Thr-24 phosphorylation, mediated by CDK1, peaks during prometaphase or metaphase cells with phosphorylated filamentous structures evident throughout the cytoplasm during early mitosis. CDK1 phosphorylates Thr-24 in mitotic cells at the site of injury. O-glycosylated. |
Function | Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress. Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes. |
Protein Name | Keratin - Type Ii Cytoskeletal 5 58 Kda Cytokeratin Cytokeratin-5 Ck-5 Keratin-5 K5 Type-Ii Keratin Kb5 |
Database Links | Reactome: R-HSA-446107 Reactome: R-HSA-6805567 Reactome: R-HSA-6809371 Reactome: R-HSA-9725554 |
Cellular Localisation | Cytoplasm |
Alternative Antibody Names | Anti-Keratin - Type Ii Cytoskeletal 5 antibody Anti-58 Kda Cytokeratin antibody Anti-Cytokeratin-5 antibody Anti-Ck-5 antibody Anti-Keratin-5 antibody Anti-K5 antibody Anti-Type-Ii Keratin Kb5 antibody Anti-KRT5 antibody |
Information sourced from Uniprot.org