• Western blot analysis of extracts of mouse large intestine cells, using Cytokeratin 16 (KRT16) antibody (STJ11103629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Confocal imaging of paraffin-embedded Human skin using Cytokeratin 16 (KRT16) Rabbit monoclonal antibody (STJ11103629, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry of paraffin-embedded human esophageal cancer using Cytokeratin 16 (KRT16) rabbit monoclonal antibody (STJ11103629) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Cytokeratin 16 (KRT16) in paraffin-embedded human esophageal cancer using Cytokeratin 16 (KRT16) Rabbit monoclonal antibody (STJ11103629) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat skin using Cytokeratin 16 (KRT16) rabbit monoclonal antibody (STJ11103629) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse large intestine, using Cytokeratin 16 (KRT16) Rabbit monoclonal antibody (STJ11103629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of human skin using Cytokeratin 16 (KRT16) rabbit monoclonal antibody (STJ11103629) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse tail and ear using Cytokeratin 16 (KRT16) rabbit monoclonal antibody (STJ11103629) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Cytokeratin 16 antibody (1-100) [S9MR] (STJ11103629)

SKU:
STJ11103629

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cytokeratin 16 (KRT16) (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT16
Gene ID: 3868
Uniprot ID: K1C16_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cytokeratin 16 (KRT16) (P08779).
Immunogen Sequence: MTTCSRQFTSSSSMKGSCGI GGGIGGGSSRISSVLAGGSC RAPSTYGGGLSVSSRFSSGG ACGLGGGYGGGFSSSSSFGS GFGGGYGGGLGAGFGGGLGA
Tissue Specificity Expressed in the corneal epithelium (at protein level).
Function Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.
Protein Name Keratin - Type I Cytoskeletal 16
Cytokeratin-16
Ck-16
Keratin-16
K16
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 16 antibody
Anti-Cytokeratin-16 antibody
Anti-Ck-16 antibody
Anti-Keratin-16 antibody
Anti-K16 antibody
Anti-KRT16 antibody
Anti-KRT16A antibody

Information sourced from Uniprot.org

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