Anti-Cytochrome P450 4A antibody (50-150) [S6MR] (STJ11103076)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103076
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General Information

Short DescriptionRabbit monoclonal Cytochrome P450 4A (CYP4A11) (50-150) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS6MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolCYP4A11
Gene ID1579
Uniprot IDCP4AB_HUMAN
Immunogen Region50-150
Immunogen SequenceQFPCPPSHWLFGHIQELQQD QELQRIQKWVETFPSACPHW LWGGKVRVQLYDPDYMKVIL GRSDPKSHGSYRFLAPWIGY GLLLLNGQTWFQHRRMLTPA F
SpecificityA synthetic peptide corresponding to a sequence within amino acids 50-150 of human Cytochrome P450 4A (CYP4A11) (CYP4A11) (Q02928).

Additional Info

Tissue Specificity Expressed in liver. Expressed in S2 and S3 segments of proximal tubules in cortex and outer medulla of kidney.
Function A cytochrome P450 monooxygenase involved in the metabolism of fatty acids and their oxygenated derivatives (oxylipins). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids, the catalytic efficiency decreasing in the following order: dodecanoic > tetradecanoic > (9Z)-octadecenoic > (9Z,12Z)-octadecadienoic > hexadecanoic acid. Acts as a major omega-hydroxylase for dodecanoic (lauric) acid in liver. Participates in omega-hydroxylation of (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) to 20-hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure. Can also catalyze the oxidation of the penultimate carbon (omega-1 oxidation) of fatty acids with lower efficiency. May contribute to the degradation of saturated very long-chain fatty acids (VLCFAs) such as docosanoic acid, by catalyzing successive omega-oxidations to the corresponding dicarboxylic acid, thereby initiating chain shortening. Omega-hydroxylates (9R,10S)-epoxy-octadecanoate stereoisomer. Plays a minor role in omega-oxidation of long-chain 3-hydroxy fatty acids. Has little activity toward prostaglandins A1 and E1.
Protein Name Cytochrome P450 4a11
20-Hydroxyeicosatetraenoic Acid Synthase
20-Hete Synthase
Cyp4aii
Cypiva11
Cytochrome P-450hk-Omega
Cytochrome P450hl-Omega
Fatty Acid Omega-Hydroxylase
Lauric Acid Omega-Hydroxylase
Long-Chain Fatty Acid Omega-Monooxygenase
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-211935
Reactome: R-HSA-211958
Reactome: R-HSA-211979
Reactome: R-HSA-2142691
Reactome: R-HSA-2142816
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Microsome Membrane
Alternative Antibody Names Anti-Cytochrome P450 4a11 antibody
Anti-20-Hydroxyeicosatetraenoic Acid Synthase antibody
Anti-20-Hete Synthase antibody
Anti-Cyp4aii antibody
Anti-Cypiva11 antibody
Anti-Cytochrome P-450hk-Omega antibody
Anti-Cytochrome P450hl-Omega antibody
Anti-Fatty Acid Omega-Hydroxylase antibody
Anti-Lauric Acid Omega-Hydroxylase antibody
Anti-Long-Chain Fatty Acid Omega-Monooxygenase antibody
Anti-CYP4A11 antibody
Anti-CYP4A2 antibody

Information sourced from Uniprot.org

Citations

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