| Short Description : | Goat polyclonal antibody anti-Cytochrome b reductase 1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications: | Pep-ELISA/WB/IHC |
| Host: | Goat |
| Reactivity: | Human/Dog/Pig/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 35kDa and a 26+28kDa doublet in human colon lysate after 0.1µg/ml antibody staining. The detected bands were all successfully blocked by wi |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | CYBRD1 |
| Gene ID: | 79901 |
| Uniprot ID: | CYBR1_HUMAN |
| Accession Number: | NP_079119.3 |
| Immunogen Region: | C-Term |
| Immunogen Sequence: | CRNLALDEAGQRSTM |
| Specificity: | This antibody is expected to recognise isoform 1 (NP_079119.3) only. |
| Function | Plasma membrane reductase that uses cytoplasmic ascorbate as an electron donor to reduce extracellular Fe(3+) into Fe(2+). Probably functions in dietary iron absorption at the brush border of duodenal enterocytes by producing Fe(2+), the divalent form of iron that can be transported into enterocytes. It is also able to reduce extracellular monodehydro-L-ascorbate and may be involved in extracellular ascorbate regeneration by erythrocytes in blood. May also act as a ferrireductase in airway epithelial cells (Probable). May also function as a cupric transmembrane reductase. |
| Protein Name | Plasma Membrane Ascorbate-Dependent Reductase Cybrd1Cytochrome B Reductase 1Duodenal Cytochrome BFerric-Chelate Reductase 3 |
| Database Links | Reactome: R-HSA-917937 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinApical Cell MembraneLocalized At The Brush Border Of Duodenal Cells |
| Alternative Antibody Names | Anti-Plasma Membrane Ascorbate-Dependent Reductase Cybrd1 antibodyAnti-Cytochrome B Reductase 1 antibodyAnti-Duodenal Cytochrome B antibodyAnti-Ferric-Chelate Reductase 3 antibodyAnti-CYBRD1 antibodyAnti-DCYTB antibodyAnti-FRRS3 antibody |
Information sourced from Uniprot.org
