| Host: | Mouse |
| Applications: | WB |
| Reactivity: | Human/Hamster |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Cytochrome P450 7A1 (C-Term) is suitable for use in Western Blot research applications. |
| Clonality : | Monoclonal |
| Conjugation: | Unconjugated |
| Formulation: | Liquid in 0.1M Tris-Glycine (pH7.4, 150 mM NaCl) , 0.2% Sodium Azide and 50% Glycerol. |
| Purification: | Affinity purification |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-1:2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYP7A1 |
| Gene ID: | 1581 |
| Uniprot ID: | CP7A1_HUMAN |
| Immunogen Region: | C-Term |
| Specificity: | CYP7A1 Monoclonal Antibody detects endogenous levels of CYP7A1 protein. |
| Immunogen: | Purified recombinant human CYP7A1 (C-terminus) protein fragments expressed in E.coli. |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives. |
| Protein Name | Cytochrome P450 7a1 24-Hydroxycholesterol 7-Alpha-Hydroxylase Cypvii Cholesterol 7-Alpha-Hydroxylase Cholesterol 7-Alpha-Monooxygenase |
| Database Links | Reactome: R-HSA-192105 Reactome: R-HSA-193368 Reactome: R-HSA-193807 Reactome: R-HSA-1989781 Reactome: R-HSA-211976 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Membrane Protein Microsome Membrane |
| Alternative Antibody Names | Anti-Cytochrome P450 7a1 antibody Anti-24-Hydroxycholesterol 7-Alpha-Hydroxylase antibody Anti-Cypvii antibody Anti-Cholesterol 7-Alpha-Hydroxylase antibody Anti-Cholesterol 7-Alpha-Monooxygenase antibody Anti-CYP7A1 antibody Anti-CYP7 antibody |
Information sourced from Uniprot.org