| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CYP7A1 (390-504) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP7A1 |
| Gene ID: |
1581 |
| Uniprot ID: |
CP7A1_HUMAN |
| Immunogen Region: |
390-504 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 390-504 of human CYP7A1 (NP_000771.2). |
| Immunogen Sequence: |
YPQLMHLDPEIYPDPLTFKY DRYLDENGKTKTTFYCNGLK LKYYYMPFGSGATICPGRLF AIHEIKQFLILMLSYFELEL IEGQAKCPPLDQSRAGLGIL PPLNDIEFKYKFKHL |
| Tissue Specificity | Detected in liver. |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis. 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives. |
| Protein Name | Cytochrome P450 7a124-Hydroxycholesterol 7-Alpha-HydroxylaseCypviiCholesterol 7-Alpha-HydroxylaseCholesterol 7-Alpha-Monooxygenase |
| Database Links | Reactome: R-HSA-192105Reactome: R-HSA-193368Reactome: R-HSA-193807Reactome: R-HSA-1989781Reactome: R-HSA-211976 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMicrosome Membrane |
| Alternative Antibody Names | Anti-Cytochrome P450 7a1 antibodyAnti-24-Hydroxycholesterol 7-Alpha-Hydroxylase antibodyAnti-Cypvii antibodyAnti-Cholesterol 7-Alpha-Hydroxylase antibodyAnti-Cholesterol 7-Alpha-Monooxygenase antibodyAnti-CYP7A1 antibodyAnti-CYP7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
