| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CYP46A1 (24-280) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP46A1 |
| Gene ID: |
10858 |
| Uniprot ID: |
CP46A_HUMAN |
| Immunogen Region: |
24-280 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 24-280 of human CYP46A1 (NP_006659.1). |
| Immunogen Sequence: |
HRARSRYEHIPGPPRPSFLL GHLPCFWKKDEVGGRVLQDV FLDWAKKYGPVVRVNVFHKT SVIVTSPESVKKFLMSTKYN KDSKMYRALQTVFGERLFGQ GLVSECNYERWHKQRRVIDL AFSRSSLVSLMETFNEKAEQ LVEILEAKADGQTPVSMQDM LTYTAMDILAKAAFGMETSM LLGAQKPLSQAVKLMLEGIT ASRNTLAKFLPGKRKQLREV RESIRFLRQVGRDWVQRRR |
| Tissue Specificity | Expressed in brain. The mRNA was broadly distributed with higher levels in gray matter zones and lower levels in regions rich in white matter. Not detected in fetal sample but its expression increases linearly with age. |
| Function | P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation. By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning. Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis. Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain. May also catalyze the oxidative metabolism of xenobiotics, such as clotrimazole. |
| Protein Name | Cholesterol 24-HydroxylaseCh24hCholesterol 24-MonooxygenaseCholesterol 24s-HydroxylaseCytochrome P450 46a1 |
| Database Links | Reactome: R-HSA-193775Reactome: R-HSA-211976 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMicrosome MembranePostsynapsePresynapseCell ProjectionDendrite |
| Alternative Antibody Names | Anti-Cholesterol 24-Hydroxylase antibodyAnti-Ch24h antibodyAnti-Cholesterol 24-Monooxygenase antibodyAnti-Cholesterol 24s-Hydroxylase antibodyAnti-Cytochrome P450 46a1 antibodyAnti-CYP46A1 antibodyAnti-CYP46 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
