| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYP46A1 |
| Gene ID: | 10858 |
| Uniprot ID: | CP46A_HUMAN |
| Immunogen Region: | 24-280 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 24-280 of human CYP46A1 (NP_006659.1). |
| Immunogen Sequence: | HRARSRYEHIPGPPRPSFLL GHLPCFWKKDEVGGRVLQDV FLDWAKKYGPVVRVNVFHKT SVIVTSPESVKKFLMSTKYN KDSKMYRALQTVFGERLFGQ GLVSECNYERWHKQRRVIDL AFSRSSLVSLMETFNEKAEQ LVEILEAKADGQTPVSMQDM LTYTAMDILAKAAFGMETSM LLGAQKPLSQAVKLMLEGIT ASRNTLAKFLPGKRKQLREV RESIRFLRQVGRDWVQRRR |
| Tissue Specificity | Expressed in brain. The mRNA was broadly distributed with higher levels in gray matter zones and lower levels in regions rich in white matter. Not detected in fetal sample but its expression increases linearly with age. |
| Function | P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation. By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning. Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis. Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain. May also catalyze the oxidative metabolism of xenobiotics, such as clotrimazole. |
| Protein Name | Cholesterol 24-Hydroxylase Ch24h Cholesterol 24-Monooxygenase Cholesterol 24s-Hydroxylase Cytochrome P450 46a1 |
| Database Links | Reactome: R-HSA-193775 Reactome: R-HSA-211976 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Membrane Protein Microsome Membrane Postsynapse Presynapse Cell Projection Dendrite |
| Alternative Antibody Names | Anti-Cholesterol 24-Hydroxylase antibody Anti-Ch24h antibody Anti-Cholesterol 24-Monooxygenase antibody Anti-Cholesterol 24s-Hydroxylase antibody Anti-Cytochrome P450 46a1 antibody Anti-CYP46A1 antibody Anti-CYP46 antibody |
Information sourced from Uniprot.org