| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYP3A4 |
| Gene ID: | 1576 |
| Uniprot ID: | CP3A4_HUMAN |
| Immunogen Region: | 244-503 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 244-503 of human CYP3A4 (NP_059488.2). |
| Immunogen Sequence: | EVTNFLRKSVKRMKESRLED TQKHRVDFLQLMIDSQNSKE TESHKALSDLELVAQSIIFI FAGYETTSSVLSFIMYELAT HPDVQQKLQEEIDAVLPNKA PPTYDTVLQMEYLDMVVNET LRLFPIAMRLERVCKKDVEI NGMFIPKGVVVMIPSYALHR DPKYWTEPEKFLPERFSKKN KDNIDPYIYTPFGSGPRNCI GMRFALMNMKLALIRVLQNF SFKPCKETQIPLKLSLGGL |
| Tissue Specificity | Expressed in prostate and liver. According to some authors, it is not expressed in brain. According to others, weak levels of expression are measured in some brain locations. Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level). |
| Post Translational Modifications | Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro). |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position. Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone. Metabolizes testosterone to less biologically active 2beta- and 6beta-hydroxytestosterones. Contributes to the formation of hydroxycholesterols (oxysterols), particularly A-ring hydroxylated cholesterol at the C-4beta position, and side chain hydroxylated cholesterol at the C-25 position, likely contributing to cholesterol degradation and bile acid biosynthesis. Catalyzes bisallylic hydroxylation of polyunsaturated fatty acids (PUFA). Catalyzes the epoxidation of double bonds of PUFA with a preference for the last double bond. Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling. Plays a role in the metabolism of retinoids. Displays high catalytic activity for oxidation of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA). Further metabolizes atRA toward 4-hydroxyretinoate and may play a role in hepatic atRA clearance. Responsible for oxidative metabolism of xenobiotics. Acts as a 2-exo-monooxygenase for plant lipid 1,8-cineole (eucalyptol). Metabolizes the majority of the administered drugs. Catalyzes sulfoxidation of the anthelmintics albendazole and fenbendazole. Hydroxylates antimalarial drug quinine. Acts as a 1,4-cineole 2-exo-monooxygenase. Also involved in vitamin D catabolism and calcium homeostasis. Catalyzes the inactivation of the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)). |
| Protein Name | Cytochrome P450 3a4 1 -4-Cineole 2-Exo-Monooxygenase 1 -8-Cineole 2-Exo-Monooxygenase Albendazole Monooxygenase Sulfoxide-Forming Albendazole Sulfoxidase Cypiiia3 Cypiiia4 Cholesterol 25-Hydroxylase Cytochrome P450 3a3 Cytochrome P450 Hlp Cytochrome P450 Nf-25 Cytochrome P450-Pcn1 Nifedipine Oxidase Quinine 3-Monooxygenase |
| Database Links | Reactome: R-HSA-211945 Reactome: R-HSA-211981 Reactome: R-HSA-5423646 Reactome: R-HSA-9027307 Reactome: R-HSA-9749641 Reactome: R-HSA-9754706 Reactome: R-HSA-9757110 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Single-Pass Membrane Protein Microsome Membrane |
| Alternative Antibody Names | Anti-Cytochrome P450 3a4 antibody Anti-1 -4-Cineole 2-Exo-Monooxygenase antibody Anti-1 -8-Cineole 2-Exo-Monooxygenase antibody Anti-Albendazole Monooxygenase antibody Anti-Sulfoxide-Forming antibody Anti-Albendazole Sulfoxidase antibody Anti-Cypiiia3 antibody Anti-Cypiiia4 antibody Anti-Cholesterol 25-Hydroxylase antibody Anti-Cytochrome P450 3a3 antibody Anti-Cytochrome P450 Hlp antibody Anti-Cytochrome P450 Nf-25 antibody Anti-Cytochrome P450-Pcn1 antibody Anti-Nifedipine Oxidase antibody Anti-Quinine 3-Monooxygenase antibody Anti-CYP3A4 antibody Anti-CYP3A3 antibody |
Information sourced from Uniprot.org
