Western blot analysis of extracts of various cell lines, using CYP39A1 antibody (STJ11103364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CYP39A1 antibody (1-100) (STJ11103364)

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STJ11103364
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Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYP39A1
Gene ID: 51302
Uniprot ID: CP39A_HUMAN
Immunogen Region: 1-100
Specificity: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CYP39A1 (NP_057677.2).
Immunogen Sequence: MELISPTVIIILGCLALFLL LQRKNLRRPPCIKGWIPWIG VGFEFGKAPLEFIEKARIKY GPIFTVFAMGNRMTFVTEEE GINVFLKSKKVDFELAVQNI
Tissue Specificity Liver specific.
Function A cytochrome P450 monooxygenase involved in neural cholesterol clearance through bile acid synthesis. Catalyzes 7-alpha hydroxylation of (24S)-hydroxycholesterol, a neural oxysterol that is metabolized to bile acids in the liver. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase).
Protein Name 24-Hydroxycholesterol 7-Alpha-Hydroxylase
Cytochrome P450 39a1
Hcyp39a1
Oxysterol 7-Alpha-Hydroxylase
Database Links Reactome: R-HSA-193775
Reactome: R-HSA-211976
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Microsome Membrane
Alternative Antibody Names Anti-24-Hydroxycholesterol 7-Alpha-Hydroxylase antibody
Anti-Cytochrome P450 39a1 antibody
Anti-Hcyp39a1 antibody
Anti-Oxysterol 7-Alpha-Hydroxylase antibody
Anti-CYP39A1 antibody

Information sourced from Uniprot.org

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