• Confocal imaging of paraffin-embedded Human placenta using CYP26A1 Rabbit monoclonal antibody (STJ11103603, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Rat placenta using CYP26A1 Rabbit monoclonal antibody (STJ11103603, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of CYP26A1 in paraffin-embedded human breast using CYP26A1 Rabbit monoclonal antibody (STJ11103603) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat liver, using CYP26A1 Rabbit monoclonal antibody (STJ11103603) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CYP26A1 antibody (201-450) [S3MR] (STJ11103603)

SKU:
STJ11103603

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-CYP26A1 (201-450) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYP26A1
Gene ID: 1592
Uniprot ID: CP26A_HUMAN
Immunogen Region: 201-450
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 201-450 of human CYP26A1 (O43174).
Immunogen Sequence: AGDGDSEQQLVEAFEEMTRN LFSLPIDVPFSGLYRGMKAR NLIHARIEQNIRAKICGLRA SEAGQGCKDALQLLIEHSWE RGERLDMQALKQSSTELLFG GHETTASAATSLITYLGLYP HVLQKVREELKSKGLLCKSN QDNKLDMEILEQLKYIGCVI KETLRLNPPVPGGFRVALKT FELNGYQIPKGWNVIYSICD THDVAEIFTNKEEFNPDRFM LPHPEDASRFSFIPFGGGL
Tissue Specificity Expressed in most fetal and adult tissues with highest levels in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain. Expressed at high levels in lung, pancreas, skin and uterus (at protein level). Lower expression level is detected in spleen, kidney, intestine and adipose tissue (at protein level).
Function A cytochrome P450 monooxygenase involved in the metabolism of retinoates (RAs), the active metabolites of vitamin A, and critical signaling molecules in animals. RAs exist as at least four different isomers: all-trans-RA (atRA), 9-cis-RA, 13-cis-RA, and 9,13-dicis-RA, where atRA is considered to be the biologically active isomer, although 9-cis-RA and 13-cis-RA also have activity (Probable). Catalyzes the hydroxylation of atRA primarily at C-4 and C-18, thereby contributing to the regulation of atRA homeostasis and signaling. Hydroxylation of atRA limits its biological activity and initiates a degradative process leading to its eventual elimination (Probable). Involved in the convertion of atRA to all-trans-4-oxo-RA. Able to metabolize other RAs such as 9-cis, 13-cis and 9,13-di-cis RA. Can oxidize all-trans-13,14-dihydroretinoate (DRA) to metabolites which could include all-trans-4-oxo-DRA, all-trans-4-hydroxy-DRA, all-trans-5,8-epoxy-DRA, and all-trans-18-hydroxy-DRA. May play a role in the oxidative metabolism of xenobiotics such as tazarotenic acid.
Protein Name Cytochrome P450 26a1
Cyp26a1
Cytochrome P450 Retinoic Acid-Inactivating 1
Cytochrome P450rai
Hp450rai
Retinoic Acid 4-Hydroxylase
Retinoic Acid-Metabolizing Cytochrome
Database Links Reactome: R-HSA-211916
Reactome: R-HSA-5365859
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Microsome Membrane
Alternative Antibody Names Anti-Cytochrome P450 26a1 antibody
Anti-Cyp26a1 antibody
Anti-Cytochrome P450 Retinoic Acid-Inactivating 1 antibody
Anti-Cytochrome P450rai antibody
Anti-Hp450rai antibody
Anti-Retinoic Acid 4-Hydroxylase antibody
Anti-Retinoic Acid-Metabolizing Cytochrome antibody
Anti-CYP26A1 antibody
Anti-CYP26 antibody
Anti-P450RAI1 antibody

Information sourced from Uniprot.org

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