| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CYP1B1 (244-543) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP1B1 |
| Gene ID: |
1545 |
| Uniprot ID: |
CP1B1_HUMAN |
| Immunogen Region: |
244-543 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 244-543 of human CYP1B1 (NP_000095.2). |
| Immunogen Sequence: |
MPWLQYFPNPVRTVFREFEQ LNRNFSNFILDKFLRHCESL RPGAAPRDMMDAFILSAEKK AAGDSHGGGARLDLENVPAT ITDIFGASQDTLSTALQWLL LLFTRYPDVQTRVQAELDQV VGRDRLPCMGDQPNLPYVLA FLYEAMRFSSFVPVTIPHAT TANTSVLGYHIPKDTVVFVN QWSVNHDPLKWPNPENFDPA RFLDKDGLINKDLTSRVMIF SVGKRRCIGEELSKMQLFL |
| Tissue Specificity | Expressed in heart, brain, lung, skeletal muscle, kidney, spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes. Expressed in retinal endothelial cells and umbilical vein endothelial cells (at protein level). |
| Function | A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Exhibits catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2- and 4-hydroxy E1 and E2. Displays a predominant hydroxylase activity toward E2 at the C-4 position. Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites. May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid. Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8,9-, 11,12-, and 14,15- EpETrE, that function as lipid mediators in the vascular system. Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs). This activity is independent of cytochrome P450 reductase, NADPH, and O2. Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products. Plays an important role in retinal vascular development. Under hyperoxic O2 conditions, promotes retinal angiogenesis and capillary morphogenesis, likely by metabolizing the oxygenated products generated during the oxidative stress. Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression. |
| Protein Name | Cytochrome P450 1b1Cypib1Hydroperoxy Icosatetraenoate Dehydratase |
| Database Links | Reactome: R-HSA-211976Reactome: R-HSA-2142670Reactome: R-HSA-2142816Reactome: R-HSA-5579000 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinMicrosome MembraneMitochondrionLocated Primarily In Endoplasmic ReticulumUpon Treatment With 2378-Tetrachlorodibenzo-P-Dioxin (Tcdd)Cyp1b1 Is Also Targeted To Mitochondria |
| Alternative Antibody Names | Anti-Cytochrome P450 1b1 antibodyAnti-Cypib1 antibodyAnti-Hydroperoxy Icosatetraenoate Dehydratase antibodyAnti-CYP1B1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
