• Western blot analysis of the lysates from HeLa cells using BCOR antibody.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Cytochrome P450 19A1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from 293 cells, using Cytochrome P450 19A1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunofluorescence analysis of A549 cells, using Cytochrome P450 19A1 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of 293 cells using CYP19A1 Polyclonal Antibody diluted at 1：1000
  • Western blot analysis of various cells using CYP19A1 Polyclonal Antibody diluted at 1：1000
  • Immunofluorescence analysis of Hela cell. 1, CYP19A1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CYP19A1 antibody (221-270 aa) (STJ92555)

SKU:
STJ92555

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Aromatase (221-270 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYP19A1
Gene ID: 1588
Uniprot ID: CP19A_HUMAN
Immunogen Region: 221-270 aa
Specificity: CYP19A1 Polyclonal Antibody detects endogenous levels of CYP19A1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Cytochrome P450 19A1 at the amino acid range 221-270
Post Translational Modifications Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1).
Function A cytochrome P450 monooxygenase that catalyzes the conversion of C19 androgens, androst-4-ene-3,17-dione (androstenedione) and testosterone to the C18 estrogens, estrone and estradiol, respectively. Catalyzes three successive oxidations of C19 androgens: two conventional oxidations at C19 yielding 19-hydroxy and 19-oxo/19-aldehyde derivatives, followed by a third oxidative aromatization step that involves C1-beta hydrogen abstraction combined with cleavage of the C10-C19 bond to yield a phenolic A ring and formic acid. Alternatively, the third oxidative reaction yields a 19-norsteroid and formic acid. Converts dihydrotestosterone to delta1,10-dehydro 19-nordihydrotestosterone and may play a role in homeostasis of this potent androgen. Also displays 2-hydroxylase activity toward estrone. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR.NADPH-ferrihemoprotein reductase).
Protein Name Aromatase
Cypxix
Cytochrome P-450arom
Cytochrome P450 19a1
Estrogen Synthase
Database Links Reactome: R-HSA-193144
Reactome: R-HSA-211976
Reactome: R-HSA-5579030
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Microsome Membrane
Alternative Antibody Names Anti-Aromatase antibody
Anti-Cypxix antibody
Anti-Cytochrome P-450arom antibody
Anti-Cytochrome P450 19a1 antibody
Anti-Estrogen Synthase antibody
Anti-CYP19A1 antibody
Anti-ARO1 antibody
Anti-CYAR antibody
Anti-CYP19 antibody

Information sourced from Uniprot.org

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