| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Cholesterol Side-Chain Cleavage Enzyme-Mitochondrial (40-320) is suitable for use in Western Blot research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
S5265RM |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CYP11A1 |
| Gene ID: |
1583 |
| Uniprot ID: |
CP11A_HUMAN |
| Immunogen Region: |
40-320 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 40-320 of human CYP11A1 (NP_000772.2). |
| Immunogen Sequence: |
ISTRSPRPFNEIPSPGDNGW LNLYHFWRETGTHKVHLHHV QNFQKYGPIYREKLGNVESV YVIDPEDVALLFKSEGPNPE RFLIPPWVAYHQYYQRPIGV LLKKSAAWKKDRVALNQEVM APEATKNFLPLLDAVSRDFV SVLHRRIKKAGSGNYSGDIS DDLFRFAFESITNVIFGERQ GMLEEVVNPEAQRFIDAIYQ MFHTSVPMLNLPPDLFRLFR TKTWKDHVAAWDVIFSKAD |
| Function | A cytochrome P450 monooxygenase that catalyzes the side-chain hydroxylation and cleavage of cholesterol to pregnenolone, the precursor of most steroid hormones. Catalyzes three sequential oxidation reactions of cholesterol, namely the hydroxylation at C22 followed with the hydroxylation at C20 to yield 20R,22R-hydroxycholesterol that is further cleaved between C20 and C22 to yield the C21-steroid pregnenolone and 4-methylpentanal. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin). |
| Protein Name | Cholesterol Side-Chain Cleavage Enzyme - MitochondrialCypxia1Cholesterol DesmolaseCytochrome P450 11a1Cytochrome P450(Scc |
| Database Links | Reactome: R-HSA-196108Reactome: R-HSA-211976Reactome: R-HSA-5579026 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinLocalizes To The Matrix Side Of The Mitochondrion Inner Membrane |
| Alternative Antibody Names | Anti-Cholesterol Side-Chain Cleavage Enzyme - Mitochondrial antibodyAnti-Cypxia1 antibodyAnti-Cholesterol Desmolase antibodyAnti-Cytochrome P450 11a1 antibodyAnti-Cytochrome P450(Scc antibodyAnti-CYP11A1 antibodyAnti-CYP11A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
