| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Goat polyclonal antibody anti-Cyb5r4-Mouse (444-458) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:64000. WB: Approx 65kDa band observed in Rat Spleen lysates (calculated MW of 55kDa according to NP_596918.2 and 58.8kDa according to Q68EJ0-1). Recommended concentration: 0.1-0.3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Immunogen Region: | 444-458 |
| Accession Number: | NP_077157.2 |
| Immunogen Sequence: | RCQLEKLALREKRFD |
| Function | NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS). |
| Protein Name | Cytochrome B5 Reductase 4 Flavohemoprotein B5/B5r B5+B5r N-Terminal Cytochrome B5 And Cytochrome B5 Oxidoreductase Domain-Containing Protein Cb5/Cb5r |
| Database Links | Reactome: R-MMU-1237044 |
| Cellular Localisation | Endoplasmic Reticulum Soluble Protein |
| Alternative Antibody Names | Anti-Cytochrome B5 Reductase 4 antibody Anti-Flavohemoprotein B5/B5r antibody Anti-B5+B5r antibody Anti-N-Terminal Cytochrome B5 And Cytochrome B5 Oxidoreductase Domain-Containing Protein antibody Anti-Cb5/Cb5r antibody Anti-Cyb5r4 antibody Anti-Ncb5or antibody |
Information sourced from Uniprot.org
