• Western blot analysis of lysates from wild type (WT) and CYB5R3 knockdown (KD) HeLa cells using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.
  • Western blot analysis of various lysates using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-CYB5R3 antibody (27-301) (STJ11105448)

SKU:
STJ11105448

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nadh-Cytochrome B5 Reductase 3 (27-301) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CYB5R3
Gene ID: 1727
Uniprot ID: NB5R3_HUMAN
Immunogen Region: 27-301
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 27-301 of human CYB5R3 (NM_000398.7).
Immunogen Sequence: FQRSTPAITLESPDIKYPLR LIDREIISHDTRRFRFALPS PQHILGLPVGQHIYLSARID GNLVVRPYTPISSDDDKGFV DLVIKVYFKDTHPKFPAGGK MSQYLESMQIGDTIEFRGPS GLLVYQGKGKFAIRPDKKSN PIIRTVKSVGMIAGGTGITP MLQVIRAIMKDPDDHTVCHL LFANQTEKDILLRPELEELR NKHSARFKLWYTLDRAPEAW DYGQGFVNEEMIRDHLPPP
Tissue Specificity Isoform 2: Expressed at late stages of erythroid maturation.
Function Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
Protein Name Nadh-Cytochrome B5 Reductase 3
B5r
Cytochrome B5 Reductase
Diaphorase-1
Database Links Reactome: R-HSA-196836
Reactome: R-HSA-211945
Reactome: R-HSA-6798695
Cellular Localisation Isoform 1: Endoplasmic Reticulum Membrane
Lipid-Anchor
Cytoplasmic Side
Mitochondrion Outer Membrane
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Nadh-Cytochrome B5 Reductase 3 antibody
Anti-B5r antibody
Anti-Cytochrome B5 Reductase antibody
Anti-Diaphorase-1 antibody
Anti-CYB5R3 antibody
Anti-DIA1 antibody

Information sourced from Uniprot.org

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