![Western blot analysis of lysates from wild type (WT) and CYB5R3 knockdown (KD) HeLa cells using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178092/370252/STJ11105448_1__45300.1713136185.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CYB5R3 knockdown (KD) HeLa cells using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.")
![Western blot analysis of various lysates using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/178092/370253/STJ11105448_2__43956.1713136186.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.")
![Western blot analysis of lysates from wild type (WT) and CYB5R3 knockdown (KD) HeLa cells using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/178092/370252/STJ11105448_1__45300.1713136185.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CYB5R3 knockdown (KD) HeLa cells using [KD Validated] CYB5R3 Rabbit polyclonal antibody (STJ11105448) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.")
| Short Description : | Rabbit polyclonal Nadh-Cytochrome B5 Reductase 3 (27-301) antibody for use in WB and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CYB5R3 |
| Gene ID: | 1727 |
| Uniprot ID: | NB5R3_HUMAN |
| Immunogen Region: | 27-301 |
| Immunogen Sequence: | FQRSTPAITLESPDIKYPLR LIDREIISHDTRRFRFALPS PQHILGLPVGQHIYLSARID GNLVVRPYTPISSDDDKGFV DLVIKVYFKDTHPKFPAGGK MSQYLESMQIGDTIEFRGPS GLLVYQGKGKFAIRPDKKSN PIIRTVKSVGMIAGGTGITP MLQVIRAIMKDPDDHTVCHL LFANQTEKDILLRPELEELR NKHSARFKLWYTLDRAPEAW DYGQGFVNEEMIRDHLPPP |
| Specificity: | Recombinant Protein corresponding to a sequence within amino acids 27-301 of human CYB5R3 (NM_000398.7). |
| Tissue Specificity | Isoform 2: Expressed at late stages of erythroid maturation. |
| Function | Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor. |
| Protein Name | Nadh-Cytochrome B5 Reductase 3B5rCytochrome B5 ReductaseDiaphorase-1 |
| Database Links | Reactome: R-HSA-196836Reactome: R-HSA-211945Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: Endoplasmic Reticulum MembraneLipid-AnchorCytoplasmic SideMitochondrion Outer MembraneIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Nadh-Cytochrome B5 Reductase 3 antibodyAnti-B5r antibodyAnti-Cytochrome B5 Reductase antibodyAnti-Diaphorase-1 antibodyAnti-CYB5R3 antibodyAnti-DIA1 antibody |
Information sourced from Uniprot.org
