Anti-CYB5R3 antibody (125-334) (STJ29673)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CYB5R3 (125-334) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CYB5R3 |
| Gene ID | 1727 |
| Uniprot ID | NB5R3_HUMAN |
| Immunogen Region | 125-334 |
| Immunogen Sequence | RPYTPISSDDDKGFVDLVIK VYFKDTHPKFPAGGKMSQYL ESMQIGDTIEFRGPSGLLVY QGKGKFAIRPDKKSNPIIRT VKSVGMIAGGTGITPMLQVI RAIMKDPDDHTVCHLLFANQ TEKDILLRPELEELRNKHSA RFKLWYTLDRAPEAWDYGQG FVNEEMIRDHLPPPEEEPLV LMCGPPPMIQYACLPNLDHV GHPTERCFVF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 125-334 of human CYB5R3 (NP_001165131.1). |
Additional Info
| Tissue Specificity | Isoform 2: Expressed at late stages of erythroid maturation. |
| Function | Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor. |
| Protein Name | Nadh-Cytochrome B5 Reductase 3B5rCytochrome B5 ReductaseDiaphorase-1 |
| Database Links | Reactome: R-HSA-196836Reactome: R-HSA-211945Reactome: R-HSA-6798695 |
| Cellular Localisation | Isoform 1: Endoplasmic Reticulum MembraneLipid-AnchorCytoplasmic SideMitochondrion Outer MembraneIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Nadh-Cytochrome B5 Reductase 3 antibodyAnti-B5r antibodyAnti-Cytochrome B5 Reductase antibodyAnti-Diaphorase-1 antibodyAnti-CYB5R3 antibodyAnti-DIA1 antibody |
Information sourced from Uniprot.org