| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CXCR2 (260-359) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CXCR2 |
| Gene ID: |
3579 |
| Uniprot ID: |
CXCR2_HUMAN |
| Immunogen Region: |
260-359 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 260-359 of human CXCR2 (NP_001548.1). |
| Immunogen Sequence: |
FLLCWLPYNLVLLADTLMRT QVIQETCERRNHIDRALDAT EILGILHSCLNPLIYAFIGQ KFRHGLLKILAIHGLISKDS LPKDSRPSFVGSSSGHTSTT |
| Post Translational Modifications | Phosphorylated upon ligand binding.which is required for desensitization. (Microbial infection) Proteolytically cleaved by Staphylococcus aureus staphopain A/SspP. This cleavage inhibits CXCR2-dependent neutrophil activation and chemotaxis. |
| Function | Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. |
| Protein Name | C-X-C Chemokine Receptor Type 2Cxc-R2Cxcr-2Cdw128bGro/Mgsa ReceptorHigh Affinity Interleukin-8 Receptor BIl-8r BIl-8 Receptor Type 2Cd Antigen Cd182 |
| Database Links | Reactome: R-HSA-380108Reactome: R-HSA-418594Reactome: R-HSA-6798695 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-C-X-C Chemokine Receptor Type 2 antibodyAnti-Cxc-R2 antibodyAnti-Cxcr-2 antibodyAnti-Cdw128b antibodyAnti-Gro/Mgsa Receptor antibodyAnti-High Affinity Interleukin-8 Receptor B antibodyAnti-Il-8r B antibodyAnti-Il-8 Receptor Type 2 antibodyAnti-Cd Antigen Cd182 antibodyAnti-CXCR2 antibodyAnti-IL8RB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89689/358177/STJ11100751_1__56006.1713122486.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NANS knockout (KO) 293T cells, using [KO Validated] NANS Rabbit polyclonal antibody (STJ11100751) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunofluorescence analysis of MCF7 cells using [KD Validated] SCD Rabbit polyclonal antibody (STJ11105577) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/178221/370505/STJ11105577_1__69759.1713136460.jpg?c=1 "Immunofluorescence analysis of MCF7 cells using [KD Validated] SCD Rabbit polyclonal antibody (STJ11105577) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
