• Western blot analysis of THP-1, using CXCL10/IP-10 antibody (STJ11100596) at 1:3000 dilution. THP-1 cells were treated by LPS for 6 hours, Brefeldin A for 3 hours and IFN Gamma (50ng/ml) for 8 hours of stimulation. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of NIH/3T3 cells using CXCL10/IP-10 rabbit polyclonal antibody (STJ11100596) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-CXCL10 antibody (22-98) (STJ11100596)

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STJ11100596

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CXCL10 (22-98) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CXCL10
Gene ID: 3627
Uniprot ID: CXL10_HUMAN
Immunogen Region: 22-98
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-98 of human CXCL10/IP-10 (NP_001556.2).
Immunogen Sequence: VPLSRTVRCTCISISNQPVN PRSLEKLEIIPASQFCPRVE IIATMKKKGEKRCLNPESKA IKNLLKAVSKERSKRSP
Tissue Specificity Mainly secreted by monocytes, endothelial cells as well as fibroblasts. Expressed by epithelial cells in thymus. Microglial cells produce CXCL10 in response to viral stimulation.
Post Translational Modifications Several proteases can mediate post-secretion cleavages. DPP4 cleaves CXCL10 on its N-terminal 2 amino acids leading to an antagonist form of CXCL10. This dominant negative form is capable of binding CXCR3 but does not induce signaling. MMP9 cleaves 9 amino acids instead.
Function Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization.
Protein Name C-X-C Motif Chemokine 10
10 Kda Interferon Gamma-Induced Protein
Gamma-Ip10
Ip-10
Small-Inducible Cytokine B10 Cleaved Into - Cxcl10(1-73
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-418594
Reactome: R-HSA-6783783
Cellular Localisation Secreted
Alternative Antibody Names Anti-C-X-C Motif Chemokine 10 antibody
Anti-10 Kda Interferon Gamma-Induced Protein antibody
Anti-Gamma-Ip10 antibody
Anti-Ip-10 antibody
Anti-Small-Inducible Cytokine B10 Cleaved Into - Cxcl10(1-73 antibody
Anti-CXCL10 antibody
Anti-INP10 antibody
Anti-SCYB10 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance