• Confocal imaging of Hep G2 cells using Cathepsin B Rabbit monoclonal antibody (STJ11104983, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of lysates from Hep G2 cells, using Cathepsin B Rabbit monoclonal antibody (STJ11104983) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Enhanced Kit. Exposure time: 120s.

Anti-CTSB antibody (8-339) [S4983RM] (STJ11104983)

SKU:
STJ11104983

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Cathepsin B (8-339) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4983RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTSB
Gene ID: 1508
Uniprot ID: CATB_HUMAN
Immunogen Region: 8-339
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 8-339 of human Cathepsin B.
Immunogen Sequence: CCLLVLANARSRPSFHPLSD ELVNYVNKRNTTWQAGHNFY NVDMSYLKRLCGTFLGGPKP PQRVMFTEDLKLPASFDARE QWPQCPTIKEIRDQGSCGSC WAFGAVEAISDRICIHTNAH VSVEVSAEDLLTCCGSMCGD GCNGGYPAEAWNFWTRKGLV SGGLYESHVGCRPYSIPPCE HHVNGSRPPCTGEGDTPKCS KICEPGYSPTYKQDKHYGYN SYSVSNSEKDIMAEIYKNG
Tissue Specificity Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.
Function Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.
Protein Name Cathepsin B
App Secretase
Apps
Cathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1679131
Reactome: R-HSA-2022090
Reactome: R-HSA-2132295
Reactome: R-HSA-6798695
Cellular Localisation Lysosome
Melanosome
Secreted
Extracellular Space
Apical Cell Membrane
Peripheral Membrane Protein
Extracellular Side
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Localizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells
Alternative Antibody Names Anti-Cathepsin B antibody
Anti-App Secretase antibody
Anti-Apps antibody
Anti-Cathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain antibody
Anti-CTSB antibody
Anti-CPSB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance