• Confocal imaging of paraffin-embedded Rat pancreas tissue using CTRL Rabbit monoclonal antibody (STJ11103074, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Mouse pancreas tissue using CTRL Rabbit monoclonal antibody (STJ11103074, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Human pancreas tissue using CTRL Rabbit monoclonal antibody (STJ11103074, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of CTRL in paraffin-embedded rat pancreas using CTRL Rabbit monoclonal antibody (STJ11103074) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CTRL in paraffin-embedded mouse pancreas using CTRL Rabbit monoclonal antibody (STJ11103074) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CTRL Rabbit monoclonal antibody (STJ11103074) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CTRL antibody (165-264) [S4MR] (STJ11103074)

SKU:
STJ11103074

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:5000-1:8000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTRL
Gene ID: 1506
Uniprot ID: CTRL_HUMAN
Immunogen Region: 165-264
Specificity: A synthetic peptide corresponding to a sequence within amino acids 165-264 of human CTRL (P40313).
Immunogen Sequence: GVGNVTPAHLQQVALPLVTV NQCRQYWGSSITDSMICAGG AGASSCQGDSGGPLVCQKGN TWVLIGIVSWGTKNCNVRAP AVYTRVSKFSTWINQVIAYN
Protein Name Chymotrypsin-Like Protease Ctrl-1
Alternative Antibody Names Anti-Chymotrypsin-Like Protease Ctrl-1 antibody
Anti-CTRL antibody
Anti-CTRL1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance