• Immunofluorescence analysis of L929 cells using CTNNA3 Rabbit polyclonal antibody (STJ118946) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of CTNNA3 in paraffin-embedded Human liver cancer using CTNNA3 Rabbit polyclonal antibody (STJ118946) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CTNNA3 in paraffin-embedded Rat testis using CTNNA3 Rabbit polyclonal antibody (STJ118946) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of CTNNA3 in paraffin-embedded Mouse testis using CTNNA3 Rabbit polyclonal antibody (STJ118946) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using CTNNA3 Rabbit polyclonal antibody (STJ118946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CTNNA3 antibody (1-240) (STJ118946)

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STJ118946

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTNNA3 (1-240) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTNNA3
Gene ID: 29119
Uniprot ID: CTNA3_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human CTNNA3 (NP_001278062.1).
Immunogen Sequence: MKSTQRREKQGSMSAETPIT LNIDPQDLQVQTFTVEKLLE PLIIQVTTLVNCPQNPSSRK KGRSKRASVLLASVEEATWN LLDKGEKIAQEATVLKDELT ASLEEVRKESEALKVSAERF TDDPCFLPKREAVVQAARAL LAAVTRLLILADMIDVMCLL QHVSAFQRTFESLKNVANKS DLQKTYQKLGKELENLDYLA FKRQQDLKSPNQRDEIAGAR ASLKENSPLLHSICSACLE
Tissue Specificity Predominantly expressed in heart and testis. Expressed at lower levels in brain, kidney, liver and skeletal muscle.
Function May be involved in formation of stretch-resistant cell-cell adhesion complexes.
Protein Name Catenin Alpha-3
Alpha T-Catenin
Cadherin-Associated Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Junction
Desmosome
Localizes To Intercalated Disks Of Cardiomyocytes And In Peritubular Myoid Cells Of Testis
And Colocalizes With Ctnna1 And Ctnna2
Colocalizes With Pkp2 At Intercalated Disks In The Heart
Alternative Antibody Names Anti-Catenin Alpha-3 antibody
Anti-Alpha T-Catenin antibody
Anti-Cadherin-Associated Protein antibody
Anti-CTNNA3 antibody

Information sourced from Uniprot.org

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