| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Chicken/Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Alpha 1-Catenin (N-Term) is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Antigen Affinity Purified |
| Dilution Range: | WB 1:1000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CTNNA1 |
| Gene ID: | 1495 |
| Uniprot ID: | CTNA1_HUMAN |
| Immunogen Region: | N-Term |
| Specificity: | The antibody detects a 102 kDa* protein corresponding to the molecular mass of Alpha 1-Catenin on SDS-PAGE immunoblots of rat PC12 and mouse SYF cells. |
| Immunogen: | Alpha 1-Catenin synthetic peptide (coupled to KLH) corresponding to amino acid residues from the N-terminal region of human Alpha 1-Catenin. This peptide sequence is highly conserved in rat and mouse Alpha 1-Catenin, and has some homology to Alpha 2- |
| Tissue Specificity | Isoform 1: Ubiquitously expressed in normal tissues. Isoform 3: Abundantly expressed in brain and cerebellum, also expressed in the placenta, liver, lung, colon, heart, pancreas, stomach and thymus. |
| Post Translational Modifications | Sumoylated. Phosphorylation seems to contribute to the strength of cell-cell adhesion rather than to the basic capacity for cell-cell adhesion. |
| Function | Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. Involved in the regulation of WWTR1/TAZ, YAP1 and TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation. May play a crucial role in cell differentiation. |
| Protein Name | Catenin Alpha-1 Alpha E-Catenin Cadherin-Associated Protein Renal Carcinoma Antigen Ny-Ren-13 |
| Database Links | Reactome: R-HSA-418990 Reactome: R-HSA-5218920 Reactome: R-HSA-525793 Reactome: R-HSA-5626467 Reactome: R-HSA-9762292 Reactome: R-HSA-9764302 Reactome: R-HSA-9833576 |
| Cellular Localisation | Cytoplasm Cytoskeleton Cell Junction Adherens Junction Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Nucleus Found At Cell-Cell Boundaries And Probably At Cell-Matrix Boundaries Isoform 3: Cell Membrane |
| Alternative Antibody Names | Anti-Catenin Alpha-1 antibody Anti-Alpha E-Catenin antibody Anti-Cadherin-Associated Protein antibody Anti-Renal Carcinoma Antigen Ny-Ren-13 antibody Anti-CTNNA1 antibody |
Information sourced from Uniprot.org