| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CTLA4 (49-223) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CTLA4 |
| Gene ID: |
1493 |
| Uniprot ID: |
CTLA4_HUMAN |
| Immunogen Region: |
49-223 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 49-223 of human CD152/CTLA-4 (NP_005205.2). |
| Immunogen Sequence: |
SSRGIASFVCEYASPGKATE VRVTVLRQADSQVTEVCAAT YMMGNELTFLDDSICTGTSS GNQVNLTIQGLRAMDTGLYI CKVELMYPPPYYLGIGNGTQ IYVIDPEPCPDSDFLLWILA AVSSGLFFYSFLLTAVSLSK MLKKRSPLTTGVYVKMPPTE PECEKQFQPYFIPIN |
| Tissue Specificity | Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation. |
| Post Translational Modifications | N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. |
| Function | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Protein Name | Cytotoxic T-Lymphocyte Protein 4Cytotoxic T-Lymphocyte-Associated Antigen 4Ctla-4Cd Antigen Cd152 |
| Database Links | Reactome: R-HSA-389513Reactome: R-HSA-8877330 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization |
| Alternative Antibody Names | Anti-Cytotoxic T-Lymphocyte Protein 4 antibodyAnti-Cytotoxic T-Lymphocyte-Associated Antigen 4 antibodyAnti-Ctla-4 antibodyAnti-Cd Antigen Cd152 antibodyAnti-CTLA4 antibodyAnti-CD152 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
