• Western blot analysis of extracts of THP1 cells, using CD152/CTLA-4 antibody (STJ115901) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CTLA4 antibody (1-100) (STJ115901)

SKU:
STJ115901

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CTLA4 (1-100) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CTLA4
Gene ID: 1493
Uniprot ID: CTLA4_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD152/CTLA-4 (NP_005205.2).
Immunogen Sequence: MACLGFQRHKAQLNLATRTW PCTLLFFLLFIPVFCKAMHV AQPAVVLASSRGIASFVCEY ASPGKATEVRVTVLRQADSQ VTEVCAATYMMGNELTFLDD
Tissue Specificity Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
Post Translational Modifications N-glycosylation is important for dimerization. Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface.
Function Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Protein Name Cytotoxic T-Lymphocyte Protein 4
Cytotoxic T-Lymphocyte-Associated Antigen 4
Ctla-4
Cd Antigen Cd152
Database Links Reactome: R-HSA-389513
Reactome: R-HSA-8877330
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Exists Primarily An Intracellular Antigen Whose Surface Expression Is Tightly Regulated By Restricted Trafficking To The Cell Surface And Rapid Internalization
Alternative Antibody Names Anti-Cytotoxic T-Lymphocyte Protein 4 antibody
Anti-Cytotoxic T-Lymphocyte-Associated Antigen 4 antibody
Anti-Ctla-4 antibody
Anti-Cd Antigen Cd152 antibody
Anti-CTLA4 antibody
Anti-CD152 antibody

Information sourced from Uniprot.org

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