| Host: |
Rabbit |
| Applications: |
WB/IF/IP |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CTH (1-405) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IF/ICC 1:50-1:200IP 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CTH |
| Gene ID: |
1491 |
| Uniprot ID: |
CGL_HUMAN |
| Immunogen Region: |
1-405 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-405 of human Cystathionase/Cystathionase/CTH (NP_001893.2). |
| Immunogen Sequence: |
MQEKDASSQGFLPHFQHFAT QAIHVGQDPEQWTSRAVVPP ISLSTTFKQGAPGQHSGFEY SRSGNPTRNCLEKAVAALDG AKYCLAFASGLAATVTITHL LKAGDQIICMDDVYGGTNRY FRQVASEFGLKISFVDCSKI KLLEAAITPETKLVWIETPT NPTQKVIDIEGCAHIVHKHG DIILVVDNTFMSPYFQRPLA LGADISMYSATKYMNGHSDV VMGLVSVNCESLHNRLRFL |
| Tissue Specificity | Highly expressed in liver. Also in muscle and lower expression in most tissues except heart, pituitary gland, spleen, thymus, and vascular tissue, where it is hardly detected. |
| Function | Catalyzes the last step in the trans-sulfuration pathway from L-methionine to L-cysteine in a pyridoxal-5'-phosphate (PLP)-dependent manner, which consists on cleaving the L,L-cystathionine molecule into L-cysteine, ammonia and 2-oxobutanoate. Part of the L-cysteine derived from the trans-sulfuration pathway is utilized for biosynthesis of the ubiquitous antioxidant glutathione. Besides its role in the conversion of L-cystathionine into L-cysteine, it utilizes L-cysteine and L-homocysteine as substrates (at much lower rates than L,L-cystathionine) to produce the endogenous gaseous signaling molecule hydrogen sulfide (H2S). In vitro, it converts two L-cysteine molecules into lanthionine and H2S, also two L-homocysteine molecules to homolanthionine and H2S, which can be particularly relevant under conditions of severe hyperhomocysteinemia (which is a risk factor for cardiovascular disease, diabetes, and Alzheimer's disease). Lanthionine and homolanthionine are structural homologs of L,L-cystathionine that differ by the absence or presence of an extra methylene group, respectively. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function. By generating the gasotransmitter H2S, it participates in a number of physiological processes such as vasodilation, bone protection, and inflammation (Probable). Plays an essential role in myogenesis by contributing to the biogenesis of H2S in skeletal muscle tissue. Can also accept homoserine as substrate. Catalyzes the elimination of selenocystathionine (which can be derived from the diet) to yield selenocysteine, ammonia and 2-oxobutanoate. |
| Protein Name | Cystathionine Gamma-LyaseCglCseCysteine DesulfhydraseCysteine-Protein SulfhydraseGamma-CystathionaseHomocysteine Desulfhydrase |
| Database Links | Reactome: R-HSA-1614558Reactome: R-HSA-1614603Reactome: R-HSA-2408508 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Cystathionine Gamma-Lyase antibodyAnti-Cgl antibodyAnti-Cse antibodyAnti-Cysteine Desulfhydrase antibodyAnti-Cysteine-Protein Sulfhydrase antibodyAnti-Gamma-Cystathionase antibodyAnti-Homocysteine Desulfhydrase antibodyAnti-CTH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
