• Western blot analysis of extracts of various cell lines, using CSTF1 antibody (STJ27711) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CSTF1 antibody (1-220) (STJ27711)

SKU:
STJ27711

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CSTF1 (1-220) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSTF1
Gene ID: 1477
Uniprot ID: CSTF1_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human CSTF1 (NP_001315.1).
Immunogen Sequence: MYRTKVGLKDRQQLYKLIIS QLLYDGYISIANGLINEIKP QSVCAPSEQLLHLIKLGMEN DDTAVQYAIGRSDTVAPGTG IDLEFDADVQTMSPEASEYE TCYVTSHKGPCRVATYSRDG QLIATGSADASIKILDTERM LAKSAMPIEVMMNETAQQNM ENHPVIRTLYDHVDEVTCLA FHPTEQILASGSRDYTLKLF DYSKPSAKRAFKYIQEAEML
Post Translational Modifications The N-terminus is blocked.
Function One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. May be responsible for the interaction of CSTF with other factors to form a stable complex on the pre-mRNA.
Protein Name Cleavage Stimulation Factor Subunit 1
Cf-1 50 Kda Subunit
Cleavage Stimulation Factor 50 Kda Subunit
Cstf 50 Kda Subunit
Cstf-50
Database Links Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77595
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cleavage Stimulation Factor Subunit 1 antibody
Anti-Cf-1 50 Kda Subunit antibody
Anti-Cleavage Stimulation Factor 50 Kda Subunit antibody
Anti-Cstf 50 Kda Subunit antibody
Anti-Cstf-50 antibody
Anti-CSTF1 antibody

Information sourced from Uniprot.org

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