| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CSRP3 (1-194) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CSRP3 |
| Gene ID: |
8048 |
| Uniprot ID: |
CSRP3_HUMAN |
| Immunogen Region: |
1-194 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human CSRP3 (NP_003467.1). |
| Immunogen Sequence: |
MPNWGGGAKCGACEKTVYHA EEIQCNGRSFHKTCFHCMAC RKALDSTTVAAHESEIYCKV CYGRRYGPKGIGYGQGAGCL STDTGEHLGLQFQQSPKPAR SVTTSNPSKFTAKFGESEKC PRCGKSVYAAEKVMGGGKPW HKTCFRCAICGKSLESTNVT DKDGELYCKVCYAKNFGPTG IGFGGLTQQVEKKE |
| Tissue Specificity | Cardiac and slow-twitch skeletal muscles. Isoform 2 is expressed in striated muscle. Isoform 2 is specifically expressed at higher levels in patients with neuromuscular diseases, such as limb-girdle muscular dystrophy 2A (LGMD2A), Duchenne muscular dystrophy (DMD) and dermatomyositis. |
| Post Translational Modifications | Phosphorylated by PKC/PRKCA. |
| Function | Positive regulator of myogenesis. Acts as cofactor for myogenic bHLH transcription factors such as MYOD1, and probably MYOG and MYF6. Enhances the DNA-binding activity of the MYOD1:TCF3 isoform E47 complex and may promote formation of a functional MYOD1:TCF3 isoform E47:MEF2A complex involved in myogenesis. Plays a crucial and specific role in the organization of cytosolic structures in cardiomyocytes. Could play a role in mechanical stretch sensing. May be a scaffold protein that promotes the assembly of interacting proteins at Z-line structures. It is essential for calcineurin anchorage to the Z line. Required for stress-induced calcineurin-NFAT activation. The role in regulation of cytoskeleton dynamics by association with CFL2 is reported conflictingly: Shown to enhance CFL2-mediated F-actin depolymerization dependent on the CSRP3:CFL2 molecular ratio, and also shown to reduce the ability of CLF1 and CFL2 to enhance actin depolymerization. Proposed to contribute to the maintenance of muscle cell integrity through an actin-based mechanism. Can directly bind to actin filaments, cross-link actin filaments into bundles without polarity selectivity and protect them from dilution- and cofilin-mediated depolymerization.the function seems to involve its self-association. In vitro can inhibit PKC/PRKCA activity. Proposed to be involved in cardiac stress signaling by down-regulating excessive PKC/PRKCA signaling. Isoform 2: May play a role in early sarcomere organization. Overexpression in myotubes negatively regulates myotube differentiation. By association with isoform 1 and thus changing the CSRP3 isoform 1:CFL2 stoichiometry is proposed to down-regulate CFL2-mediated F-actin depolymerization. |
| Protein Name | Cysteine And Glycine-Rich Protein 3Cardiac Lim ProteinCysteine-Rich Protein 3Crp3Lim Domain Protein - CardiacMuscle Lim Protein |
| Cellular Localisation | NucleusCytoplasmCytoskeletonMyofibrilSarcomereZ LineNucleocytoplasmic Shuttling ProteinMainly CytoplasmicIn The Z LineFound Associated With Glrx3Isoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Cysteine And Glycine-Rich Protein 3 antibodyAnti-Cardiac Lim Protein antibodyAnti-Cysteine-Rich Protein 3 antibodyAnti-Crp3 antibodyAnti-Lim Domain Protein - Cardiac antibodyAnti-Muscle Lim Protein antibodyAnti-CSRP3 antibodyAnti-CLP antibodyAnti-MLP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
