![Immunofluorescence analysis of HeLa cells using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101475/389282/STJ28096_1__59763.1713158942.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and CSNK1E knockout (KO) HeLa cells, using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101475/389283/STJ28096_2__30517.1713158943.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and CSNK1E knockout (KO) HeLa cells, using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of various lysates using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101475/389284/STJ28096_3__41155.1713158943.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunofluorescence analysis of HeLa cells using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/101475/389282/STJ28096_1__59763.1713158942.jpg?c=1 "Immunofluorescence analysis of HeLa cells using [KO Validated] CSNK1E Rabbit polyclonal antibody (STJ28096). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-CSNK1E antibody (247-416) (STJ28096)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CSNK1E (247-416) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CSNK1E |
| Gene ID | 1454 |
| Uniprot ID | KC1E_HUMAN |
| Immunogen Region | 247-416 |
| Immunogen Sequence | EFSTYLNFCRSLRFDDKPDY SYLRQLFRNLFHRQGFSYDY VFDWNMLKFGAARNPEDVDR ERREHEREERMGQLRGSATR ALPPGPPTGATANRLRSAAE PVASTPASRIQPAGNTSPRA ISRVDRERKVSMRLHRGAPA NVSSSDLTGRQEVSRIPASQ TSVPFDHLGK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1). |
Additional Info
| Tissue Specificity | Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes. |
| Post Translational Modifications | Autophosphorylated. Partially dephosphorylated by PPP5C. May be dephosphorylated by PP1. |
| Function | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates (Probable). Participates in Wnt signaling. Phosphorylates DVL1. Phosphorylates DVL2. Phosphorylates NEDD9/HEF1. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation. |
| Protein Name | Casein Kinase I Isoform EpsilonCki-EpsilonCkie |
| Database Links | Reactome: R-HSA-201688Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-400253Reactome: R-HSA-5620912Reactome: R-HSA-6791226Reactome: R-HSA-8854518 |
| Cellular Localisation | CytoplasmNucleus |
| Alternative Antibody Names | Anti-Casein Kinase I Isoform Epsilon antibodyAnti-Cki-Epsilon antibodyAnti-Ckie antibodyAnti-CSNK1E antibody |
Information sourced from Uniprot.org