• Immunohistochemistry analysis of paraffin-embedded mouse spleen using CSF3 antibody (STJ27939) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using CSF3 antibody (STJ27939) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using CSF3 antibody (STJ27939) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using CSF3 antibody (STJ27939) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CSF3 antibody (30-207) (STJ27939)

SKU:
STJ27939

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CSF3 (30-207) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CSF3
Gene ID: 1440
Uniprot ID: CSF3_HUMAN
Immunogen Region: 30-207
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-207 of human CSF3 (NP_000750.1).
Immunogen Sequence: ATPLGPASSLPQSFLLKCLE QVRKIQGDGAALQEKLVSEC ATYKLCHPEELVLLGHSLGI PWAPLSSCPSQALQLAGCLS QLHSGLFLYQGLLQALEGIS PELGPTLDTLQLDVADFATT IWQQMEELGMAPALQPTQGA MPAFASAFQRRAGGVLVASH LQSFLEVSYRVLRHLAQP
Post Translational Modifications O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
Function Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
Protein Name Granulocyte Colony-Stimulating Factor
G-Csf
Pluripoietin
Filgrastim
Lenograstim
Database Links Reactome: R-HSA-449836
Reactome: R-HSA-6783783
Reactome: R-HSA-9674555
Reactome: R-HSA-9705462
Cellular Localisation Secreted
Alternative Antibody Names Anti-Granulocyte Colony-Stimulating Factor antibody
Anti-G-Csf antibody
Anti-Pluripoietin antibody
Anti-Filgrastim antibody
Anti-Lenograstim antibody
Anti-CSF3 antibody
Anti-C17orf33 antibody
Anti-GCSF antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance