| Host: |
Mouse |
| Applications: |
WB/FC/ICC/IP |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-CREB-regulated transcription coactivator 1 is suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
3D7-E5-D9 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2b |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:1000ICC 1:300FCM 1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CRTC1 |
| Gene ID: |
23373 |
| Uniprot ID: |
CRTC1_HUMAN |
| Specificity: |
This antibody detects endogenous levels of MECT1/Torc1 and does not cross-react with related proteins. |
| Immunogen: |
Purified recombinant human MECT1/Torc1 protein fragments expressed in E.coli. |
| Post Translational Modifications | Phosphorylation/dephosphorylation states of Ser-151 are required for regulating transduction of CREB activity. TORCs are inactive when phosphorylated, and active when dephosphorylated at this site. This primary site of phosphorylation is mediated by SIKs (SIK1 and SIK2), is regulated by cAMP and calcium levels and is dependent on the phosphorylation of SIKs by LKB1. |
| Function | Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. In the hippocampus, involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. May be required for dendritic growth of developing cortical neurons. In concert with SIK1, regulates the light-induced entrainment of the circadian clock. In response to light stimulus, coactivates the CREB-mediated transcription of PER1 which plays an important role in the photic entrainment of the circadian clock. (Microbial infection) Plays a role of coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). |
| Protein Name | Creb-Regulated Transcription Coactivator 1Mucoepidermoid Carcinoma Translocated Protein 1Transducer Of Regulated Camp Response Element-Binding Protein 1Torc-1Transducer Of Creb Protein 1 |
| Database Links | Reactome: R-HSA-2151201Reactome: R-HSA-400253Reactome: R-HSA-9707616 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic When Phosphorylated By Sik Or Ampk And When Sequestered By 14-3-3 ProteinsTranslocated To The Nucleus On Ser-151 DephosphorylationInstigated By A Number Of Factors Including Calcium Ion And Camp LevelsLight Stimulation Triggers A Nuclear Accumulation In The Suprachiasmatic Nucleus (Scn) Of The Brain |
| Alternative Antibody Names | Anti-Creb-Regulated Transcription Coactivator 1 antibodyAnti-Mucoepidermoid Carcinoma Translocated Protein 1 antibodyAnti-Transducer Of Regulated Camp Response Element-Binding Protein 1 antibodyAnti-Torc-1 antibodyAnti-Transducer Of Creb Protein 1 antibodyAnti-CRTC1 antibodyAnti-KIAA0616 antibodyAnti-MECT1 antibodyAnti-TORC1 antibodyAnti-WAMTP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
