• Immunohistochemistry analysis of paraffin-embedded mouse lung using CRTAP antibody (STJ23231) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using CRTAP antibody (STJ23231) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using CRTAP antibody (STJ23231) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of SW480 cells, using CRTAP antibody (STJ23231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-CRTAP antibody (20-240) (STJ23231)

SKU:
STJ23231

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRTAP
Gene ID: 10491
Uniprot ID: CRTAP_HUMAN
Immunogen Region: 20-240
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human CRTAP (NP_006362.1).
Immunogen Sequence: ALRAGRAQYERYSFRSFPRD ELMPLESAYRHALDKYSGEH WAESVGYLEISLRLHRLLRD SEAFCHRNCSAAPQPEPAAG LASYPELRLFGGLLRRAHCL KRCKQGLPAFRQSQPSREVL ADFQRREPYKFLQFAYFKAN NLPKAIAAAHTFLLKHPDDE MMKRNMAYYKSLPGAEDYIK DLETKSYESLFIRAVRAYNG ENWRTSITDMELALPDFFKA F
Tissue Specificity Found in articular chondrocytes. Expressed in a variety of tissues.
Function Necessary for efficient 3-hydroxylation of fibrillar collagen prolyl residues.
Protein Name Cartilage-Associated Protein
Database Links Reactome: R-HSA-1650814
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Cartilage-Associated Protein antibody
Anti-CRTAP antibody
Anti-CASP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance