| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S7MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CRK |
| Gene ID: | 1398 |
| Uniprot ID: | CRK_HUMAN |
| Immunogen Region: | 1-100 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CRK/CRKII (P46108). |
| Immunogen Sequence: | MAGNFDSEERSSWYWGRLSR QEAVALLQGQRHGVFLVRDS STSPGDYVLSVSENSRVSHY IINSSGPRPPVPPSPAQPPP GVSPSRLRIGDQEFDSLPAL |
| Post Translational Modifications | Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. Isoform Crk-II: Phosphorylated by KIT. Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form. |
| Function | Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
| Protein Name | Adapter Molecule CrkProto-Oncogene C-CrkP38 |
| Database Links | Reactome: R-HSA-170984Reactome: R-HSA-186763Reactome: R-HSA-2029482Reactome: R-HSA-372708Reactome: R-HSA-4420097Reactome: R-HSA-8849471Reactome: R-HSA-8875555Reactome: R-HSA-8875656Reactome: R-HSA-912631Reactome: R-HSA-9664422 |
| Cellular Localisation | CytoplasmCell MembraneTranslocated To The Plasma Membrane Upon Cell Adhesion |
| Alternative Antibody Names | Anti-Adapter Molecule Crk antibodyAnti-Proto-Oncogene C-Crk antibodyAnti-P38 antibodyAnti-CRK antibody |
Information sourced from Uniprot.org
