Anti-CRISPR Cas9 antibody (C-Term) [6F7] (STJA0003839)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenRecombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.
General Information
| Short Description | Mouse monoclonal anti-CRISPR Cas9 (C-Term) for use in WB, IHC and ICC in samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/ICC |
| Host | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 6F7 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | This antibody was protein g purified culture from supernatant. |
| Dilution Range | WB 1:1000IHC 1:1000-1:5000ICC 1:500-1:5000 |
| Formulation | 100 ul in PBS + 50% Glycerol and 5 mM Sodium Azide |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Immunogen | Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus. |
| Immunogen Region | C-Term |
Additional Info
| Background | CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015). |
Information sourced from Uniprot.org