| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-CRBN (1-280) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
CRBN |
| Gene ID: |
51185 |
| Uniprot ID: |
CRBN_HUMAN |
| Immunogen Region: |
1-280 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human CRBN (NP_057386.2?). |
| Immunogen Sequence: |
MAGEGDQQDAAHNMGNHLPL LPAESEEEDEMEVEDQDSKE AKKPNIINFDTSLPTSHTYL GADMEEFHGRTLHDDDSCQV IPVLPQVMMILIPGQTLPLQ LFHPQEVSMVRNLIQKDRTF AVLAYSNVQEREAQFGTTAE IYAYREEQDFGIEIVKVKAI GRQRFKVLELRTQSDGIQQA KVQILPECVLPSTMSAVQLE SLNKCQIFPSKPVSREDQCS YKWWQKYQKRKFHCANLTS |
| Tissue Specificity | Widely expressed. Highly expressed in brain. |
| Post Translational Modifications | Ubiquitinated, ubiquitination is mediated by its own DCX protein ligase complex. |
| Function | Substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins, such as MEIS2 or ILF2. Normal degradation of key regulatory proteins is required for normal limb outgrowth and expression of the fibroblast growth factor FGF8. Maintains presynaptic glutamate release and consequently cognitive functions, such as memory and learning, by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. Likely to function by regulating the assembly and neuronal surface expression of BK channels via its interaction with KCNT1. May also be involved in regulating anxiety-like behaviors via a BK channel-independent mechanism. Plays a negative role in TLR4 signaling by interacting with TRAF6 and ECSIT, leading to inhibition of ECSIT ubiquitination, an important step of the signaling. |
| Protein Name | Protein Cereblon |
| Database Links | Reactome: R-HSA-9679191 |
| Cellular Localisation | CytoplasmNucleusMembranePeripheral Membrane Protein |
| Alternative Antibody Names | Anti-Protein Cereblon antibodyAnti-CRBN antibodyAnti-AD-006 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
