• Immunofluorescence analysis of RD cells using CRAT Rabbit polyclonal antibody (STJ28448) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CRAT antibody (STJ28448) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CRAT antibody (380-626) (STJ28448)

SKU:
STJ28448

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CRAT (380-626) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRAT
Gene ID: 1384
Uniprot ID: CACP_HUMAN
Immunogen Region: 380-626
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 380-626 of human CRAT (NP_000746.3).
Immunogen Sequence: KLRFNITPEIKSDIEKAKQN LSIMIQDLDITVMVFHHFGK DFPKSEKLSPDAFIQMALQL AYYRIYGQACATYESASLRM FHLGRTDTIRSASMDSLTFV KAMDDSSVTEHQKVELLRKA VQAHRGYTDRAIRGEAFDRH LLGLKLQAIEDLVSMPDIFM DTSYAIAMHFHLSTSQVPAK TDCVMFFGPVVPDGYGVCYN PMEAHINFSLSAYNSCAETN AARLAHYLEKALLDMRALL
Tissue Specificity Mostly in skeletal muscle, less in heart, liver and pancreas, only weakly detectable in brain, placenta, lung and kidney.
Function Catalyzes the reversible transfer of acyl groups from carnitine to coenzyme A (CoA) and regulates the acyl-CoA/CoA ratio. Also plays a crucial role in the transport of fatty acids for beta-oxidation. Responsible for the synthesis of short- and branched-chain acylcarnitines. Active towards some branched-chain amino acid oxidation pathway (BCAAO) intermediates. Trans-2-enoyl-CoAs and 2-methylacyl-CoAs are poor substrates.
Protein Name Carnitine O-Acetyltransferase
Carnitine Acetylase
Carnitine Acetyltransferase
Cat
Crat
Database Links Reactome: R-HSA-389887
Reactome: R-HSA-9033241
Cellular Localisation Endoplasmic Reticulum
Peroxisome
Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Isoform 1: Mitochondrion
Isoform 2: Peroxisome
Alternative Antibody Names Anti-Carnitine O-Acetyltransferase antibody
Anti-Carnitine Acetylase antibody
Anti-Carnitine Acetyltransferase antibody
Anti-Cat antibody
Anti-Crat antibody
Anti-CRAT antibody
Anti-CAT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance