• Western blot analysis of extracts of various cell lines, using RAIDD/CRADD rabbit monoclonal antibody (STJ11102349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Immunohistochemistry analysis of paraffin-embedded human breast using RAIDD/CRADD Rabbit monoclonal antibody (STJ11102349) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human breast using RAIDD/CRADD rabbit monoclonal antibody (STJ11102349) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using RAIDD/CRADD Rabbit monoclonal antibody (STJ11102349) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-CRADD antibody (70-150) [S9MR] (STJ11102349)

SKU:
STJ11102349

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-RAIDD (70-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CRADD
Gene ID: 8738
Uniprot ID: CRADD_HUMAN
Immunogen Region: 70-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 70-150 of human CRADD (P78560).
Immunogen Sequence: DTFLDSLQEFPWVREKLKKA REEAMTDLPAGDRLTGIPSH ILNSSPSDRQINQLAQRLGP EWEPMVLSLGLSQTDIYRCK A
Tissue Specificity Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.
Function Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis. Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway.
Protein Name Death Domain-Containing Protein Cradd
Caspase And Rip Adapter With Death Domain
Rip-Associated Protein With A Death Domain
Database Links Reactome: R-HSA-6803207
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Death Domain-Containing Protein Cradd antibody
Anti-Caspase And Rip Adapter With Death Domain antibody
Anti-Rip-Associated Protein With A Death Domain antibody
Anti-CRADD antibody
Anti-RAIDD antibody

Information sourced from Uniprot.org

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