• Western blot analysis of extracts of various cell lines, using CR2/CD21 antibody (STJ110705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of mouse spleen using CR2/CD21 Polyclonal Antibody (STJ110705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat spleen using CR2/CD21 antibody (STJ110705) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of human spleen using CR2/CD21 Polyclonal Antibody (STJ110705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen using CR2/CD21 Polyclonal Antibody (STJ110705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen using CR2/CD21 Polyclonal Antibody (STJ110705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human spleen using CR2/CD21 Polyclonal Antibody (STJ110705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using CR2/CD21 antibody (STJ110705) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse spleen using CR2/CD21 Polyclonal Antibody (STJ110705) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CR2/CD21 antibody (STJ110705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CR2 antibody (300-590) (STJ110705)

SKU:
STJ110705

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:100
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CR2
Gene ID: 1380
Uniprot ID: CR2_HUMAN
Immunogen Region: 300-590
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human CR2/CD21 (NP_001006659.1).
Immunogen Sequence: IVTYTCDPDPEEGVNFILIG ESTLRCTVDSQKTGTWSGPA PRCELSTSAVQCPHPQILRG RMVSGQKDRYTYNDTVIFAC MFGFTLKGSKQIRCNAQGTW EPSAPVCEKECQAPPNILNG QKEDRHMVRFDPGTSIKYSC NPGYVLVGEESIQCTSEGVW TPPVPQCKVAACEATGRQLL TKPQHQFVRPDVNSSCGEGY KLSGSVYQECQGTIPWFMEI RLCKEITCPPPPVIYNGAH
Tissue Specificity Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
Function Serves as a receptor for various ligands including complement component CD3d, HNRNPU OR IFNA1. When C3d is bound to antigens, attaches to C3d on B-cell surface and thereby facilitates the recognition and uptake of antigens by B-cells. This interaction enhances B-cell activation and subsequent immune responses. Forms a complex with several partners on the surface of B-cells including CD19, FCRL5 and CD81, to form the B-cell coreceptor complex that plays a crucial role in B-cell activation and signaling. Induces also specific intracellular signaling separately from the BCR and CD19 by activating the tyrosine kinase SRC, which then phosphorylates nucleolin/NCL and triggers AKT and GSK3 kinase activities in a SYK/CD19-independent manner. Acts as a ligand for CD23 (FcepsilonRII), a low-affinity receptor for IgE, which is expressed on B-cells and other immune cells, and thus participates in the regulation of IgE production. (Microbial infection) Acts as a receptor for Epstein-Barr virus.
Protein Name Complement Receptor Type 2
Cr2
Complement C3d Receptor
Epstein-Barr Virus Receptor
Ebv Receptor
Cd Antigen Cd21
Database Links Reactome: R-HSA-977606
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Complement Receptor Type 2 antibody
Anti-Cr2 antibody
Anti-Complement C3d Receptor antibody
Anti-Epstein-Barr Virus Receptor antibody
Anti-Ebv Receptor antibody
Anti-Cd Antigen Cd21 antibody
Anti-CR2 antibody
Anti-C3DR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance