• Western blot analysis of HeLa, using CPT1A rabbit polyclonal antibody (STJ27260) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using CPT1A rabbit polyclonal antibody (STJ27260) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of HeLa cells using CPT1A rabbit polyclonal antibody (STJ27260) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using CPT1A rabbit polyclonal antibody (STJ27260) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of MCF7 cells using 3 Mu g CPT1A antibody (STJ27260). Western blot was performed from the immunoprecipitate using CPT1A antibody (STJ27260) at a dilution of 1:1000.
  • Anti-CPT1A antibody (520-720) (STJ27260)

Anti-CPT1A antibody (520-720) (STJ27260)

SKU:
STJ27260

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CPT1A (520-720) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CPT1A
Gene ID: 1374
Uniprot ID: CPT1A_HUMAN
Immunogen Region: 520-720
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 520-720 of human CPT1A (NP_001867.2).
Immunogen Sequence: WDIPGECQEVIETSLNTANL LANDVDFHSFPFVAFGKGII KKCRTSPDAFVQLALQLAHY KDMGKFCLTYEASMTRLFRE GRTETVRSCTTESCDFVRAM VDPAQTVEQRLKLFKLASEK HQHMYRLAMTGSGIDRHLFC LYVVSKYLAVESPFLKEVLS EPWRLSTSQTPQQQVELFDL ENNPEYVSSGGGFGPVADDG Y
Tissue Specificity Strong expression in kidney and heart, and lower in liver and skeletal muscle.
Function Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in hepatic triglyceride metabolism.
Protein Name Carnitine O-Palmitoyltransferase 1 - Liver Isoform
Cpt1-L
Carnitine O-Palmitoyltransferase I - Liver Isoform
Cpt I
Cpti-L
Carnitine Palmitoyltransferase 1a
Database Links Reactome: R-HSA-1368082
Reactome: R-HSA-1989781
Reactome: R-HSA-200425
Reactome: R-HSA-5362517
Cellular Localisation Mitochondrion Outer Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Carnitine O-Palmitoyltransferase 1 - Liver Isoform antibody
Anti-Cpt1-L antibody
Anti-Carnitine O-Palmitoyltransferase I - Liver Isoform antibody
Anti-Cpt I antibody
Anti-Cpti-L antibody
Anti-Carnitine Palmitoyltransferase 1a antibody
Anti-CPT1A antibody
Anti-CPT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance