• Western blot analysis of extracts of various cell lines, using CPSF2 antibody (STJ111646) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Western blot analysis of various lysates using CPSF2 Rabbit polyclonal antibody (STJ111646) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CPSF2 antibody (450-700) (STJ111646)

SKU:
STJ111646

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CPSF2 (450-700) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CPSF2
Gene ID: 53981
Uniprot ID: CPSF2_HUMAN
Immunogen Region: 450-700
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 450-700 of human CPSF2 (NP_059133.1).
Immunogen Sequence: KGSFFKQAKKSYPMFPAPEE RIKWDEYGEIIKPEDFLVPE LQATEEEKSKLESGLTNGDE PMDQDLSDVPTKCISTTESI EIKARVTYIDYEGRSDGDSI KKIINQMKPRQLIIVHGPPE ASQDLAECCRAFGGKDIKVY MPKLHETVDATSETHIYQVR LKDSLVSSLQFCKAKDAELA WIDGVLDMRVSKVDTGVILE EGELKDDGEDSEMQVEAPSD SSVIAQQKAMKSLFGDDEK
Function Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.
Protein Name Cleavage And Polyadenylation Specificity Factor Subunit 2
Cleavage And Polyadenylation Specificity Factor 100 Kda Subunit
Cpsf 100 Kda Subunit
Database Links Reactome: R-HSA-159231
Reactome: R-HSA-72187
Reactome: R-HSA-72203
Reactome: R-HSA-73856
Reactome: R-HSA-77595
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cleavage And Polyadenylation Specificity Factor Subunit 2 antibody
Anti-Cleavage And Polyadenylation Specificity Factor 100 Kda Subunit antibody
Anti-Cpsf 100 Kda Subunit antibody
Anti-CPSF2 antibody
Anti-CPSF100 antibody
Anti-KIAA1367 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance