• Immunofluorescence analysis of PC-12 cells using COX5B Rabbit polyclonal antibody (STJ23208) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using COX5B Rabbit polyclonal antibody (STJ23208) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of COX5B in paraffin-embedded human liver tissue using COX5B Rabbit polyclonal antibody (STJ23208) at a dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COX5B in paraffin-embedded human liver cancer tissue using COX5B Rabbit polyclonal antibody (STJ23208) at a dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of COX5B in paraffin-embedded human colon tissue using COX5B Rabbit polyclonal antibody (STJ23208) at a dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using COX5B Rabbit polyclonal antibody (STJ23208) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-COX5B antibody (32-129) (STJ23208)

SKU:
STJ23208

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:100
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: COX5B
Gene ID: 1329
Uniprot ID: COX5B_HUMAN
Immunogen Region: 32-129
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2).
Immunogen Sequence: ASGGGVPTDEEQATGLEREI MLAAKKGLDPYNVLAPKGAS GTREDPNLVPSISNKRIVGC ICEEDNTSVVWFWLHKGEAQ RCPRCGAHYKLVPQQLAH
Function Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
Protein Name Cytochrome C Oxidase Subunit 5b - Mitochondrial
Cytochrome C Oxidase Polypeptide Vb
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-611105
Reactome: R-HSA-9707564
Reactome: R-HSA-9837999
Reactome: R-HSA-9864848
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Cytochrome C Oxidase Subunit 5b - Mitochondrial antibody
Anti-Cytochrome C Oxidase Polypeptide Vb antibody
Anti-COX5B antibody

Information sourced from Uniprot.org

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