• STJ70093 (0. 01µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • STJ70093 (0. 1µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
  • STJ70093 (0. 03µg/ml) staining of HEK293 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Anti-Cortactin/EMS1 antibody (N-Term) (STJ70093)

SKU:
STJ70093-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Cortactin/EMS1 (N-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: CTTN
Gene ID: 2017
Uniprot ID: SRC8_HUMAN
Immunogen Region: N-Term
Accession Number: NP_005222.2; NP_612632.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_005222.2; NP_612632.1).
Immunogen Sequence: WKASAGHAVSIA
Function Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones. Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density. Plays a role in focal adhesion assembly and turnover. In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits. Required for stabilization of KCNH1 channels at the cell membrane. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
Protein Name Src Substrate Cortactin
Amplaxin
Oncogene Ems1
Database Links Reactome: R-HSA-5627123
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Lamellipodium
Ruffle
Dendrite
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Podosome
Cell Junction
Focal Adhesion
Membrane
Clathrin-Coated Pit
Dendritic Spine
Cell Cortex
Endoplasmic Reticulum
Colocalizes Transiently With Ptk2/Fak1 At Focal Adhesions
Associated With Membrane Ruffles And Lamellipodia
In The Presence Of Cttnbp2nl
Colocalizes With Stress Fibers
In The Presence Of Cttnbp2
Localizes At The Cell Cortex
In Response To Neuronal Activation By Glutamate
Redistributes From Dendritic Spines To The Dendritic Shaft
Colocalizes With Dnm2 At The Basis Of Filopodia In Hippocampus Neuron Growth Zones
Alternative Antibody Names Anti-Src Substrate Cortactin antibody
Anti-Amplaxin antibody
Anti-Oncogene Ems1 antibody
Anti-CTTN antibody
Anti-EMS1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance